Modeling Real|Time PCR Kinetics

Mathematical model of real-time PCR kinetics - Gene-Quantification

Abstract: Several real-time PCR (rtPCR) quantification techniques are currently used to determine the expression levels of individual genes from rtPCR data ...

Mathematical model of real-time PCR kinetics - PubMed

We have developed a mathematical model of the annealing and extension phases of the PCR process. Using the model, we can simulate the PCR process over a series ...

Modeling Real-Time PCR Kinetics: Richards Reparametrized ...

Abstract ... Real-time PCR is the most sensitive method for detection and precise quantification of specific DNA sequences, but it is not usually applied as a ...

Modeling real-time PCR kinetics: Richards reparametrized equation ...

Modeling real-time PCR kinetics: Richards reparametrized equation for quantitative estimation of European hake (Merluccius merluccius) · Authors.

A kinetic-based sigmoidal model for the polymerase chain reaction ...

Based upon defining a common reference point, current real-time quantitative PCR technologies compare relative differences in amplification ...

Mathematical model of real‐time PCR kinetics - Wiley Online Library

Abstract Several real-time PCR (rtPCR) quantification techniques are currently used to determine the expression levels of individual genes ...

Mathematical model of real-time PCR kinetics. - Semantic Scholar

A mathematical model of the annealing and extension phases of the PCR process is developed that allows us to predict the efficiency of rtPCR at any cycle ...

Validation of a quantitative method for real time PCR kinetics

Mathematical Model of Real-Time PCR Kinetics | Request PDF

A study proposed a mathematical model of PCR kinetics curve, they simulated the PCR process over a series of reaction cycles which allows for predicting the ...

Validation of a quantitative method for real time PCR kinetics

Real time RT-PCR is the most sensitive method for quantitation of gene expression levels. The accuracy can be dependent on the mathematical model on which ...

Mathematics of quantitative kinetic PCR and the application of ...

Kinetic PCR (kPCR) allows quantification of a target DNA within a sample, with the advantage that sensitivity is independent of copy number (1–4). The key ...

A STOCHASTIC MODEL AND SIMULATION ALGORITHM FOR ...

A new stochastic approach to model Polymerase Chain. Reaction (PCR) kinetic is presented, in which primer and template DNA sequences, enzyme concentration,.

Stepwise kinetic equilibrium models of quantitative polymerase ...

It is argued that the kinetic approach to modeling and interpreting quantitative PCR ... Model of Real-Time PCR Kinetics. Biotechnol Bioeng 2005, ...

Mathematical model of real-time PCR kinetics - Academia.edu

Immediately after the Persian Wars, several Athenians already started to resume the practice of dedicating statues and statue groups on the Athenian Acropolis, ...

Mathematical Modeling of PCR with Variable Concentrations of DNA ...

Springer. 7. Gevertz, J.L., S.M. Dunn, and C.M. Roth, Mathematical model of real‐time PCR kinetics. Biotechnology and bioengineering, 2005 ...

Modeling of PCR: Kinetic Explanation for Short‐Chained Side ...

A simple mathematical model based on step growth processes to describe the polymerase chain reaction (PCR) is introduced.

Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification ...

We describe a simple, quantitative assay for any amplifiable DNA sequence that uses a video camera to monitor multiple polymerase chain ...

new mathematical model for relative quantification in real-time RT ...

Use of the real-time polymerase chain reaction (PCR) to amplify cDNA ... ) Kinetic PCR analysis: real-time monitoring of DNA amplification reactions.

Robust and Precise Quantitative Real-Time Polymerase Chain ...

Robust and Precise Quantitative Real-Time Polymerase Chain Reaction with an Amplification Efficiency-Aware Reaction Kinetics Model. Click to ...

Analysis of PCR Kinetics inside a Microfluidic DNA Amplification ...

... polymerase chain reaction (PCR) kinetics are presented. The ... Mehra, S.; Hu, W.S. A kinetic model of quantitative real-time polymerase chain reaction.