Modification of the active centre of nattokinase to enhance ...

Modification of the active centre of nattokinase to enhance its ...

Methods: In order to solve the above problems, a new method based on molecular dynamics simulation, steered dynamics simulation and conservative ...

Modification of the active centre of nattokinase to enhance ... - Frontiers

Modification of the active centre of nattokinase to enhance its thermostability using a strategy based on molecular dynamics simulation, steered dynamics ...

Modification of the active centre of nattokinase to enhance its ...

Modification of the active centre of nattokinase to enhance its thermostability using a strategy based on molecular dynamics simulation, ...

Data_Sheet_1_Modification of the active centre of nattokinase to ...

Data_Sheet_1_Modification of the active centre of nattokinase to enhance its thermostability using a strategy based on molecular dynamics ...

Improving the activity of the subtilisin nattokinase by site-directed ...

Nattokinase (NK), a bacterial serine protease from Bacillus subtilis var. natto, is a potential cardiovascular drug exhibiting strong fibrinolytic activity. To ...

Combined Computer-Aided Predictors to Improve the ... - PubMed

Food-derived nattokinase has strong thrombolytic activity ... Local structure analysis showed that the increased rigidity of the active center ...

Nattokinase as a functional food ingredient: therapeutic applications ...

NK has shown excellent anti-thrombus, thrombolytic, and anti-inflammation activities that potentially delay aging and provide therapeutic effects on aging- ...

Enhanced Thermostability of Nattokinase by Computation-Based ...

However, its low thermostability and fibrinolytic activity limit its application in food and pharmaceuticals. In this study, we used ...

Exploring the In Vitro Thrombolytic Activity of Nattokinase From a ...

Nattokinase (NK) is a potent fibrinolytic agent for thrombosis therapy. Objectives: The aim of this study was to enhance the production of NK from Pseudomonas ...

High-Efficiency Fermentation of Nattokinase by Recombinant PSP2 ...

Ser-His-Asp (D32, H64, S221) constitutes its catalytic center. Nattokinase exhibits considerable activity at pH 5.5 to 9.0 [3,4]. Compared to ...

(PDF) Combined Computer-Aided Predictors to Improve the ...

Food-derived nattokinase has strong thrombolytic activity and few side effects. In the field of medicine, nattokinase has been developed as ...

Stepwise modifications of genetic parts reinforce the secretory ...

Nattokinase (NK) is an important serine-protease with direct fibrinolytic activity involving the prevention of cardiovascular disease as an ...

Heterologous expression of nattokinase from B. subtilis natto using ...

Although nattokinase shows high similarity to many subtilisins in the serine protease family, it is the only member with thrombolytic activity.

Three-dimensional structure of nattokinase. (A) Calcium binding site ...

The catalytically active center of nattokinase consists of a conserved catalytic triad (Asp32, His64, Ser221), while its substrate-binding center contains ...

Nattokinase: Insights into Biological Activity, Therapeutic ... - MDPI

Therefore, applying mutagenesis breeding to the modification of nattokinase-producing strains can enhance both enzyme production capacity and ...

Non-covalent modification of thrombolytic agent nattokinase

Nattokinase (NK), as a novel thrombolytic agent, has gained more and more attention. However, NK is susceptible to chemical oxidation, and subsequent ...

Nattokinase - Ortho Molecular Products

Derived from a highly purified extract of the traditional Japanese food natto, nattokinase is a natural, highly active enzyme that helps promote normal levels ...

Directed evolution improves the fibrinolytic activity of nattokinase ...

In the current report, DNA family shuffling was used to improve the fibrinolytic activity of nattokinase. Three homologous genes from B. natto ...

Nattokinase - Memorial Sloan Kettering Cancer Center

Nattokinase is an enzyme used to ferment soybeans. It is also available as a supplement and is most known for its potential effects on ...

NATTOKINASE - Uses, Side Effects, and More - WebMD

Nattokinase is an enzyme that comes from a Japanese food called natto. Natto is made from boiled soybeans that have been fermented with a type of bacteria.