Optimization of CRISPR–Cas system for clinical cancer therapy

Optimization of CRISPR-Cas system for clinical cancer therapy

In this review article, we describe the mechanism of the CRISPR-Cas systems and highlight the applications of class II Cas effector proteins.

Although autologous CAR-T cells have shown promising results in cancer treatment, there are still some barriers to overcome, particularly the extreme high cost.

Optimization of CRISPR–Cas system for clinical cancer therapy - Meng

In this review article, we describe the mechanism of the CRISPR–Cas systems and highlight the applications of class II Cas effector proteins.

Optimizing CRISPR/Cas9 precision: Mitigating off-target effects for ...

CRISPR/Cas9 precision genome editing has revolutionized cancer treatment by introducing specific alterations to the cancer genome.

CRISPR/Cas9 therapeutics: progress and prospects - Nature

Due to the limitations of the PAM, the CRISPR/Cas9 gene-editing system often fails to target the proper sites. Therefore, the modification of ...

Recent advances and applications of CRISPR-Cas9 in cancer ...

Cancer immunotherapy aims to activate the immune system in an inhibitory condition due to the TME, restoring its ability to kill tumor cells [40] ...

Engineering CRISPR/Cas9 therapeutics for cancer precision medicine

CRISPR-associated protein 9 (Cas9) systems provide one-such unique tool that enables not only to edit (e.g., add, delete, substitute) the genome ...

Application of CRISPR/Cas9 Technology in Cancer Treatment - MDPI

Gene editing's rapid advancement has increased its medical/clinical value. Due to its great specificity and efficiency, CRISPR/Cas9 can ...

Review article CRISPR/Cas9-based application for cancer therapy

coli in1987 is more flexible and efficient [7,8]. ... The primary CRISPR/Cas9 clinical trial in China is editing T cells from patients with CRISPR/Cas9 system in ...

Comprehensive review of CRISPR-based gene editing

The CRISPR system is a revolutionary genome editing tool that has the potential to revolutionize the field of cancer research and therapy.

Leveraging CRISPR gene editing technology to optimize the efficacy ...

The Cas12a/Cpf1 protein, which originates from Acidaminococcus and belongs to the Type V CRISPR-Cas system [19], is also favored in CAR T-cell ...

Optimization of CRISPR-Cas9 system in Eustoma grandiflorum

In this study, we optimized single guide RNA and Cas9 promoters of the CRISPR-Cas9 vector and established an efficient protoplast isolation and transient ...

Revolutionizing in vivo therapy with CRISPR/Cas genome editing

They demonstrated that the CRISPR/Cas9 system could be engineered to precisely target and cleave specific DNA sequences within a broad spectrum of organisms, ...

UAT: Optimization of CRISPR-Cas system for clinical cancer therapy

DSpace preserves and enables easy and open access to all types of digital content including text, images, moving images, mpegs and data sets ; Title: ...

CRISPR–Cas: a tool for cancer research and therapeutics

CRISPR systems are being robustly adapted to improve the efficacy of immunotherapies by enhancing their potency, mitigating toxicity, reducing manufacturing ...

CRISPR/Cas9 for Cancer Therapy: Hopes and Challenges - MDPI

Overall, CRISPR/Cas9 has shown an unprecedented clinical potential to study and target disease and to offer new avenues for drug discovery.

Optimization of CRISPR–Cas system for clinical cancer ...

Furthermore, effective vector delivery must be met for the CRISPR–Cas systems to enter human clinical trials. In this review article, we describe the mechanism ...

Optimization of CRISPR–Cas system for clinical cancer therapy

Optimization of CRISPR–Cas system for clinical cancer therapy. Bioeng. Transl. Med. Pub Date : 2022-12-23. DOI : 10.1002/btm2.10474. Xiang Meng 1 ...

CRISPR Clinical Trials: A 2024 Update - Innovative Genomics Institute

Lesser known than its famous cousin Cas9, Cas3 shreds DNA at the gene regions it is targeted to find. In this treatment, the CRISPR-Cas3 system ...

CRISPR-Cas9 genome editing using targeted lipid nanoparticles for ...

Harnessing CRISPR-Cas9 technology for cancer therapeutics has been hampered by low editing efficiency in tumors and potential toxicity of ...