Optimization of lentiviral vector production for scale|up in fixed|bed ...

Optimization of lentiviral vector production for scale-up in fixed-bed ...

Lentiviral vectors (LVs) are promising tools for gene therapy. However, scaling up the production methods of LVs in order to produce ...

Optimization of lentiviral vector production for scale-up in fixed-bed ...

Optimising and developing a scalable, chemically-defined, animal component-free lentiviral vector pr... ... Lentiviral vectors (LVVs) play a critical role in gene ...

Optimizing lentiviral vector production for efficient clinical-scale ...

Stirred-tank and fixed-bed systems are both being pursued for large-scale LV vector production. Employing suspension cells in stirred-tank ...

Optimizing and developing a scalable, chemically defined, animal ...

25. Valkama, A.J. ∙ Leinonen, H.M. ∙ Lipponen, E.M. ... Optimization of lentiviral vector production for scale-up in fixed-bed bioreactor.

Optimizing and developing a scalable, chemically defined, animal ...

Lentiviral vectors (LVVs) play a critical role in gene delivery for ex vivo gene-modified cell therapies. However, the lack of scalable LVV ...

Lentiviral Vector Production from a Stable Packaging Cell Line ...

By optimizing fetal bovine serum (FBS) concentration, pH post-induction, and day of induction, we attain viral yields of more than 2 × 107 transducing units/mL.

Optimization of lentiviral vector production for scale-up in fixed-bed ...

Optimization of lentiviral vector production for scale-up in fixed-bed bioreactor · List of references · Publications that cite this publication.

Fixed-bed bioreactors optimized for high-scale LV production.

The demand for LV vectors has increased with the rise in disease indications, making it crucial to improve current manufacturing processes. Upstream bioreactor ...

Lentivirus production study in the scale-X™ fixed-bed bioreactor

Overcoming the challenges in industrial-scale Lentivirus manufacturing ... Lentiviral vectors (LV) face significant challenges in industrial-scale manufacturing ...

Benchmarking of Scale-X Bioreactor System in Lentiviral and ...

Univercells' scale-X fixed-bed bioreactor has proven to be efficient for viral vector manufacturing. Cell growth was monitored by nuclei count, glucose ...

Lentiviral vector bioprocess economics for cell and gene therapy ...

Heikura, et al. Optimization of lentiviral vector production for scale-up in fixed-bed bioreactor. Gene Ther., 25 (2018), pp. 39-46. Jan. Crossref View in ...

Large-Scale Production of Lentiviral Vectors: Current Perspectives ...

LentiPro26 is capable of producing up to 1.6 × 106 TU/mL/d for more than 60 days and is scalable to HYPERflask, enabling harvesting 3.4 L of the supernatant [51] ...

Optimizing lentiviral vector formulation conditions for ... - Cytotherapy

Optimization of lentiviral vector production for scale-up in fixed-bed bioreactor. Gene Ther 2018;25:39–46. https://doi.org/10.1038/gt ...

Scale-up of lentiviral vectors for gene therapy - BioInsights

Growing interest in the use of lentiviral (LV) vectors for gene therapy applications has resulted in demand for production processes that are amenable to ...

Optimization of lentiviral vector production for scale-up in fixed-bed ...

Optimization of lentiviral vector production for scale-up in fixed-bed bioreactor. Gene therapy, 2018-01, Vol.25 (1), p.39-46 [Peer Reviewed Journal]. The ...

Optimization of lentiviral vector production for scale-up in fixed-bed ...

Although large-scale LV production can be executed in suspension conditions, adherent production is generally favored because of relatively high cell densities ...

Optimizing lentiviral vector formulation conditions for efficient ex vivo ...

The functional and viral particle titers and vector copy number were measured after LV cryopreservation and up to 24 h post-thaw incubation. CAR-Ts were ...

Optimization of lentiviral vector production for scale-up in fixed-bed ...

Optimization of lentiviral vector production for scale-up in fixed-bed bioreactor · Gene Therapy, October 2017 · 10.1038/gt.2017.91 · 29345252 · A J Valkama, H M ...

Optimizing and developing a scalable, chemically defined, animal ...

Large-scale clinical-grade retroviral vector production in a fixed-bed bioreactor. ... Optimization of lentiviral vector produc- tion for scale-up in fixed-bed ...

Optimization of lentiviral vector production - Aaltodoc

The aim of this study was to optimize the production of lentiviral vectors in a 4 m2 fixed-bed bioreactor with calcium phosphate transfection, ...