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Primary Mouse Small Intestinal Epithelial Cell Cultures


Primary mouse small intestinal epithelial cell cultures - PubMed

The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have recently shown that Lgr5 (Leucine-rich repeat-containing G ...

Primary cultures of mouse small intestinal epithelial cells using the ...

We found that a combination of collagenase and hyaluronidase was more effective in the release of many crypts within a short time, greatly reducing the ...

Generation of renewable mouse intestinal epithelial cell monolayers ...

... cell proliferation and adenoma formation in the small intestine and colon [22]. Primary cultures of intestinal epithelium from genetic mouse ...

Development of a primary mouse intestinal epithelial cell monolayer ...

There is significant interest in the use of primary intestinal epithelial cells in monolayer culture to model intestinal biology.

Primary Murine Small Intestinal Epithelial Cells, Maintained in Long ...

We describe a method for long-term culture of primary small intestinal epithelial cells (IEC) from suckling mice. IEC were digested from intestinal ...

C57BL/6 Mouse Primary Small Intestinal Epithelial Cells

C57BL/6 Mouse Primary Small Intestinal Epithelial Cells from Cell Biologics are isolated from the small intestinal tissues of pathogen-free laboratory mice.

Primary Mouse Small Intestinal Epithelial Cell Cultures - SpringerLink

Here, we describe methods that we have established for the isolation and culture of primary small intestinal epithelial stem cells.

Primary mouse small intestinal epithelial cell cultures - Keio University

Primary mouse small intestinal epithelial cell cultures. / Sato, Toshiro; Clevers, Hans. Epithelial Cell Culture Protocols: Second Edition. ed. / Scott Randell; ...

Primary mouse small intestinal epithelial cell cultures.

In this culture system, isolated crypts form "organoid structures" with a histological hierarchy recapitulating in vivo small intestinal epithelium, ...

A bioengineering perspective on modelling the intestinal epithelial ...

... cell lines but also for primary intestinal epithelial cells. New ... et al. Development of a primary mouse intestinal epithelial cell monolayer ...

Article Development and Characterization of a Human and Mouse ...

Cultures from the mouse small ... Development of a primary mouse intestinal epithelial cell monolayer culture system to evaluate factors that ...

Primary Mouse Small Intestinal Epithelial Cell Cultures

Single isolated Lgr5+ intestinal stem cells also form these organoid structures, in which stem cells are maintained by self-renewal and give rise to all ...

Primary mouse small intestinal epithelial cell cultures. - Europe PMC

Abstract. The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have recently shown that Lgr5 (Leucine-rich repeat-containing ...

BALB/c Mouse Primary Small Intestinal Epithelial Cells

Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of ...

Generation of Mouse Small Intestinal Epithelial Cell Lines ... - PLOS

Cell lines derived from the small intestine that reflect authentic properties of the originating intestinal epithelium are of high value for studies on mucosal ...

Primary Cell-Derived Intestinal Models: Recapitulating Physiology

The reverse self-organization was observed when mouse small intestine epithelial cells were cultured on a Matrigel coating: proliferative cells were ...

Primary Mouse Small Intestinal Epithelial Cell Cultures | Request PDF

Request PDF | Primary Mouse Small Intestinal Epithelial Cell Cultures | The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals.

Diabetic Mouse Small Intestinal Epithelial Cells - Cell Biologics Inc.

Diabetic Mouse Small Intestinal Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated ...

Mouse Intestinal Organoids, Cryopreserved

Cryopreserved cells enable establishment of organoid cultures without isolation of crypts from primary mouse intestinal tissue

C57BL/6 Mouse Small Intestinal Epithelial Cells - Creative Bioarray

Each vial contains at least 0.5x10^6 cells per ml and is delivered frozen. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture ...