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Protein–DNA binding


DNA-binding protein - Wikipedia

DNA-binding proteins are proteins that have DNA-binding domains and thus have a specific or general affinity for single- or double-stranded DNA. ... Sequence- ...

The structure, function and evolution of proteins that bind DNA and ...

Key Points · DNA- and RNA-binding proteins (DRBPs) constitute a significant fraction of cellular proteins and have important roles in cells.

Methods for Detecting Protein-DNA Interactions - US

The ChIP assay method allows analysis of DNA–protein interactions in living cells by treating the cells with formaldehyde or other crosslinking reagents in ...

DNA Binding - an overview | ScienceDirect Topics

DNA binding refers to the process where a protein, such as AR, attaches to DNA after undergoing a significant conformational change, which is crucial for ...

DNA-Binding Motifs in Gene Regulatory Proteins - NCBI

Gene regulatory proteins recognize short stretches of double-helical DNA of defined sequence and thereby determine which of the thousands of genes in a cell ...

Protein–DNA binding: complexities and multi-protein codes

We review the complexities in protein–DNA binding found at multiple levels and discuss how they confound the idea of simple recognition codes.

Proteins Recognizing DNA: Structural Uniqueness and Versatility of ...

Proteins use a wide range of DNA-binding structural motifs, such as homeodomain (HD), helix-turn-helix (HTH), and high-mobility group box (HMG) to recognize DNA ...

DNA-Binding Proteins: Overview & Application - Excedr

DNA-binding proteins are proteins having an affinity for different DNA molecules. They contain DNA-binding sites to recognize and bind specific ...

DNA Binding - an overview | ScienceDirect Topics

Various proteins bind in the major groove of DNA molecules and perform a specific function. Examples of DNA-binding proteins include those involved in ...

Geometric deep learning of protein–DNA binding specificity - Nature

We present Deep Predictor of Binding Specificity (DeepPBS), a geometric deep-learning model designed to predict binding specificity from protein–DNA structure.

Protein:DNA Interactions - Promega Corporation

Chromatin immunoprecipitation (ChIP) is a method used to determine whether DNA-binding proteins, such as transcription factors, associate with a specific ...

An overview of the structures of protein-DNA complexes

As the name indicates, the DNA-binding domain consists of a loop leading out of the main body of the protein, followed by a small ß sheet, an a ...

Physicochemical models of protein–DNA binding with ... - PNAS

We introduce models that directly and precisely represent physicochemical readout, which is, importantly, not restricted to standard Watson–Crick base pairs.

Protein–DNA/RNA Interactions: An Overview of Investigation ...

The present review proposes an overview of the approaches most commonly employed in proteomics applications for the identification of nucleic acid-binding ...

DNA–protein interaction studies: a historical and comparative analysis

This review intends to provide a historical context and compile the methods that describe DNA–protein interactions according to the purpose of each approach.

Protein−DNA binding in the absence of specific base-pair recognition

We identify a new protein−DNA binding mechanism induced by DNA sequence symmetry in the absence of specific base-pair recognition.

Protein–DNA binding sites prediction based on pre-trained protein ...

We propose a novel approach called Contrastive Learning And Pre-trained Encoder (CLAPE), which combines a pre-trained protein language model and the ...

DNA Interactions with Proteins - News-Medical

During the process of complex formation, hydrogen bonds between the polar amino acids and the atoms of the DNA molecule augment the affinity of ...

Protein-DNA binding: data, tools & models

JASPAR is an open-access database of curated, non-redundant transcription factor (TF) binding profiles stored as position frequency matrices ( ...

Purification or Removal of DNA-Binding Proteins - Sigma-Aldrich

Remove ionically bound proteins by washing with 0.5 CV of 2 M NaCl for 10 to 15 min. Remove precipitated or denatured proteins by washing with 4 CV of 100 mM ...