Quantification of hepatitis C virus RNA by competitive amplification ...

Abstract. The direct detection of hepatitis C virus (HCV) RNA by PCR is widely used to determine the presence of circulating virions. The most relevant limit of ...

Competitive. RT-PCR methods for the quantification of viral nucleic acid detected serum HCV RNA concentrations of between 102 and. 109 (32) and between 104 and ...

Fully automated rapid quantification of Hepatitis C Virus RNA in ...

The amplification efficiency was calculated to be 96% (PCR efficiency = 10(−1/slope) − 1). Figure 1. figure 1. Real time detection of HCV RNA by ...

Quantitation of hepatitis C virus RNA by competitive polymerase ...

To circumvent the problem of variability in efficiency of amplification, competitive PCR has been used for accurate quantitation of RNA [Andre and Hahlbrock,.

Quantitation of HCV-replication using one-step competitive reverse ...

The reduction in the amplification of competitor is dependent upon the concentration of HCV-RNA in the original sample. External hepatitis C virus wild-type ...

Real-time detection system for quantification of hepatitis C virus ...

Furthermore, the RTD-PCR is 10–100-fold more sensitive than the Amplicor monitor for detection of HCV genotype 1b and 2b synthetic RNA. Intraassay precision was ...

Quantitative analysis of hepatitis C virus RNA by competitive nested ...

A method that allows the quantitation of hepatitis C virus (HCV) RNA is described. The RNA was extracted from serum samples and reverse transcribed.

Real-time RT-PCR for quantitation of hepatitis C virus RNA

It was demonstrated that the real-time RT-PCR was able to amplify HCV RNA in reference sera with seven genotypes (1A, 1B, 2B, 3A, 4, 5A and 6A) that include six ...

Quantitative Analysis of Hepatitis C Virus RNA in Serum During ...

HCV RNA in serum was quantified by a competitive. RT-PCR assay according to ... hepatitis C virus ribonucleic acid in the serum by amplification with ...

Quantitative analysis of hepatitis C virus RNA in liver biopsies by ...

Viral RNA concentration (which ranged from 2 to 2.7 × 105 HCV-RNA molecules per 1016 β-actin molecules) directly correlated with the efficacy of treatment, ...

Molecular Diagnostics of Hepatitis C Virus Infection - JAMA Network

In addition, real-time PCR can accurately quantify HCV RNA levels over a linear range exceeding 6 logs (ie, 10 IU/mL to 100 million IU/mL) for ...

A method for near full-length amplification and sequencing for six ...

We applied this protocol to 122 HCV positive clinical samples, and had a successful genome amplification rate of 90 %, when the viral load was ...

Rapid detection of hepatitis C virus RNA by a reverse transcription ...

To determine the sensitivity of the HCV RT-LAMP assay, the HCV RNAs transcripts in vitro were used as templates and quantified by the COBAS Amplicor Monitor PCR ...

(PDF) Quantification of hepatitis C virus RNA by competitive ...

We report a technique of competitive amplification allowing the estimation of HCV RNA copy number in biological samples. We constructed a standard competitive ...

Quantification of hepatitis C virus RNA in serum by branched DNA ...

Semantic Scholar extracted view of "Quantification of hepatitis C virus RNA in serum by branched DNA-based signal amplification assays.

Comparison of Methodologies for Quantification of Hepatitis C Virus ...

Abstract. Quantification of hepatitis C virus (HCV) RNA is important in the assessment of HCV-associated liver disease in patients coinfected with HCV and.

Quantitative detection of hepatitis C virus genome in liver tissue and ...

We quantified hepatitis C virus RNA in 25 Japanese patients with chronic type C liver disease by competitive reverse transcription-polymerase chain reaction ...

(PDF) Comparison of competitive and non ... - Academia.edu

We have developed a sensitive and reproducible one-step competitive reverse transcriptase (RT) PCR assay, which allows hepatitis C virus (HCV) RNA quantitation ...

Comparison of HCV RNA assays for the detection and quantification ...

Detection and quantification of hepatitis C virus (HCV) RNA ... C virus RNA ... A technique of competitive amplification allowing the estimation of HCV RNA ...

Quantification of hepatitis C virus with homologous RNA standards ...

We developed a quantitative competitive RT-PCR assay for measuring the levels of HCV RNA in biological fluids that uses a synthetic part of HCV RNA genome as ...