Quantitation of HCV|replication using one|step competitive reverse ...

Quantitation of HCV-replication using one-step competitive reverse ...

Quantitation of HCV-replication using one-step competitive reverse transcription-polymerase chain reaction and a solid phase, colorimetric detection method.

Quantitation of HCV-replication using one-step competitive reverse ...

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Quantitation of HCV-replication using one-step competitive reverse ...

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Quantitation of HCV-replication using one-step competitive reverse transcription-polymerase chain reaction and a solid phase, colorimetric detection method.

Quantification of hepatitis C virus RNA by competitive amplification ...

The evaluation of HCV RNA titer required a single coamplification with the competitor and could be read from a standard curve. Furthermore, this method proved ...

Journal of Virological Methods - ScienceDirect.com

A competitive reverse transcription (RT)-nested polymerase chain reaction (PCR) assay for HCV RNA was compared with the Roche Amplicor HCV Monitor assay, ...

Real-time detection system for quantification of hepatitis C virus ...

Methods: Hepatitis C virus genome was detected by real/time detection system using an ABI Prism 7700 sequence detector (Perkin Elmer Corp./Applied Biosystems, ...

Comparison of HCV RNA Levels by Branched DNA Probe Assay ...

HCV RNA was grouped into four genotypes by PCR with type-specific primers,and HCV RNA level was measured by branched DNA probeassay and by competitive PCR. HCV ...

Molecular Diagnostics of Hepatitis C Virus Infection - JAMA Network

The most commonly used qualitative NATs use reverse transcription PCR to detect viral RNA as reviewed elsewhere. There are 3 widely used tests ...

Molecular characterization and dynamics of hepatitis C virus ... - aasld

tion in human fetal hepatocytes (HFHs) were addressed in this human hepatitis have been answered in the last few years study. Using a competitive reverse- ...

Quantification of Hepatitis C Virus (HCV) in Liver Specimens and ...

The virological end-of-treatment response in the serum was associated with a lower pretreatment intrahepatic HCV viral load (P = 0.03). The new version of b-DNA ...

Fully automated rapid quantification of Hepatitis C Virus RNA in ...

As the concentration of the MS2 IC has been optimized and does not interfere with HCV amplification or compromise the detection of low titers ...

Dynamics of Hepatitis C Virus Replication in Human Liver

HCV replication varied among infected hepatocytes, occurred in a subset of cells, and proceeded at a low level, confirming one mechanism by which individual ...

Quantitative Analysis of Hepatitis C Virus RNA in Serum During ...

ment using a competitive assay that combined reverse transcription and ... The threshold of the. RT-PCR assay was analyzed using synthetic mutant HCV.

Laboratory Tests and Hepatitis C - Viral Hepatitis and Liver Disease

Recombinant immunoblot assay (RIBA) is a highly specific test that in the past was used as a confirmatory test of antibody results. It still required HCV RNA ...

Determination of serum hepatitis C virus (HCV) core protein using a ...

One (7.7%) of 13 patients with HCV genotype 1 and 5 (62.8%) of 8 patients ... Quantification of hepatitis C virus by competitive reverse ...

Human cell types important for Hepatitis C Virus replication in vivo ...

For liver [25, 26] and serum [25] the ratio of positive to negative strands were estimated to be between 1 and 1000 using semi-quantitative PCR.

Assessment of Viral Loads in Patients with Chronic Hepatitis C with ...

The level and range of quantification were similar when measured with the COBAS HCV MONITOR version 2.0 and QUANTIPLEX HCV RNA version 2.0 assays, at 3.03 × 106 ...

HEPATITIS C DIAGNOSTICS TECHNOLOGY LANDSCAPE - Unitaid

The ARCHITECT HCV cAg Assay is a two-step immunoassay using CIA technology, with flexible assay protocols referred to as CHEMIFLEX, for the quantitative ...

FIG. 7. Correlation between the number of copies obtained by the...

We have developed a sensitive and reproducible one-step competitive reverse transcriptase (RT) PCR assay, which allows hepatitis C virus (HCV) RNA quantitation ...