Rapid and Specific Detection of Active SARS|CoV|2 With CRISPR ...

Rapid and Specific Detection of Active SARS-CoV-2 With CRISPR ...

Rapid and specific detection of active SARS-CoV-2 with CRISPR/Cas12a. Xinyi Liu, 1 , 2 , † Yanhua Li, 3 , † Xin Wang, 4 Yifan Song, 5 Lina Wu, 6 Benyuan Yu

Fast and sensitive detection of SARS-CoV-2 RNA using suboptimal ...

CRISPR-based assays for the detection of nucleic acids are highly specific, yet they are not fast, sensitive or easy to use.

Rapid and Accurate Detection of the SARS-CoV-2 Omicron Variant ...

In a microplate (Applied), 10 nM synthetic DNA (unless otherwise specified) was mixed with 50 nM CRISPR-Cas12a ribonucleoprotein and 500 nM ...

Rapid detection of SARS-CoV-2 with CRISPR-Cas12a | PLOS Biology

Thus, there is an urgent demand to develop a rapid, inexpensive, and sensitive diagnostic test to aid point-of-care viral detection for disease ...

CRISPR-based systems for sensitive and rapid on-site COVID-19 ...

Antigen rapid tests enable point-of-care (POC) detection but are significantly less sensitive/specific. CRISPR-Cas systems are compatible with ...

Rapid, multiplex detection of SARS-CoV-2 using isothermal ... - Nature

Here we report a fast (~ 40 min), highly sensitive, point-of-care multiplex RT-LAMP and CRISPR/Cas12a assay to detect SARS-CoV-2.

Rapid and Specific Detection of Active SARS-CoV-2 With CRISPR ...

In this study, to specifically detect active virus replication, we developed a CRISPR -based act ive SARS- CoV -2 (CRISPR-actCoV) detection strategy by ...

Ultrasensitive Rapid Detection of SARS-CoV-2 with a Cascade ...

Currently, CRISPR/Cas-based molecular diagnostic techniques usually rely on the introduction of nucleic acid amplification to improve their ...

CRISPR-based detection of SARS-CoV-2 - ScienceDirect.com

According to guidelines by WHO, key features in accessing the practicality of a POC diagnostic device are affordable, sensitive, specific, user-friendly, rapid.

CRISPR-Cas12a-Based Detection for the Major SARS-CoV-2 ...

We described CRISPR-Cas12-based multiplex allele-specific assay for rapid SARS-CoV-2 variant genotyping. The new system has the potential to ...

FnCas9-based CRISPR diagnostic for rapid and accurate detection ...

made a lateral flow test that could detect a Cas9 protein that binds to a nucleic acid sequence found in a specific mutant strain of SARS-CoV-2.

CRISPR-Cas12a-Empowered Electrochemical Biosensor for Rapid ...

Using the DNA template identical to SARS-CoV-2 Delta spike gene sequence as model, our biosensor exhibited excellent analytical detection limit ...

CRISPR is a useful biological tool for detecting nucleic acid of SARS ...

The recent pandemic of novel coronavirus disease (COVID-19) has spread globally and infected millions of people. The quick and specific detection of the ...

CRISPR/Cas12a-empowered surface plasmon resonance platform ...

A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2

Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a ...

CRISPR diagnostics can augment gold-standard PCR-based testing if they can be made rapid, portable, and accurate. Here, we report the ...

Electric field-driven microfluidics for rapid CRISPR-based ... - PNAS

We apply our method to the rapid detection of SARS-CoV-2 RNA in clinical samples. Our method takes about 35 min from raw sample to result, a ...

In Silico Evaluation of CRISPR-Based Assays for Effective Detection ...

... Specific Detection of Respiratory Pathogens Including SARS-CoV-2. ACS Chem ... Rapid Detection of SARS-CoV-2 with CRISPR-Cas12a. PLoS Biol. 2020, 18 ...

Rapid and Specific Detection of Active SARS-CoV-2 With CRISPR ...

Rapid and sensitive nucleic acid detection of SARS-CoV-2 has contributed to the clinical diagnosis and control of COVID-19. Although detection of virus ...

Digital CRISPR/Cas‐Assisted Assay for Rapid and Sensitive ...

The crucial but unmet need for rapid and sensitive nucleic acid detection assays for diagnostic testing of highly infectious diseases has become ...

CRISPR-Cas System: An Approach With Potentials for COVID-19 ...

... rapid and specific diagnosis of COVID-19. The CRISPR-Cas-based methods diagnose the SARS-CoV-2 infections within an hour. Apart from its ...