Stable Isotopes for Tracing Mammalian|Cell Metabolism in vivo

Stable Isotopes for Tracing Mammalian-Cell Metabolism In Vivo

Metabolism is at the cornerstone of all cellular functions and mounting evidence of its deregulation in different diseases emphasizes the importance of a ...

Stable Isotopes for Tracing Mammalian-Cell Metabolism In Vivo

Stable-isotope measurements are increasingly used to probe mammalian-cell metabolism in vivo. The selection of stable-isotope tracer(s) and ...

Global stable-isotope tracing metabolomics reveals system-wide ...

In summary, global in vivo isotope tracing metabolomics enabled delineation of metabolic activities with a metabolome-wide coverage and ...

13C tracer analysis suggests extensive recycling of endogenous ...

In contrast, stable isotope tracing provides information on metabolite dynamics and generates data on specific metabolic pathways. Stable ...

Physiological impact of in vivo stable isotope tracing on cancer ...

Depending on the intracellular metabolic labeling, isotope tracing helps to infer metabolite interconversion, a feature that cannot be perceived by steady state ...

Comprehensive stable-isotope tracing of glucose and amino acids ...

When a 13C-labeled substrate such as glucose or a specific amino acid is added to cell culture and subsequently metabolized, 13C-labeling from ...

In vivo isotope tracing reveals a requirement for the electron ...

Stable isotope tracing is a versatile and informative method to explore nutrient uptake and metabolism in intact biological systems, including ...

Metabolic Flux Analysis and In Vivo Isotope Tracing

Lower Sensitivity but Deeper Labeling: Stable isotope tracers offer lower sensitivity but often achieve deeper labeling. They allow simultaneous investigation ...

Stable isotope tracing to assess tumor metabolism in vivo

Metabolism within these tissues during the infusion transfers the isotope label into metabolic intermediates from pathways supplied by the infused nutrient.

Stable Isotopes for Tracing Cardiac Metabolism in Diseases - Frontiers

Especially, stable-isotope tracers allow to administer probes to a biological system (e.g., animal cells) in cost-efficient and safe way. At the same time ...

Stable isotope tracing to assess tumor metabolism in vivo

We have developed methods to use stable isotope-labeled nutrients (e.g., [13C]glucose) to probe metabolic activity within intact tumors in vivo, in mice and ...

Physiological impact of in vivo stable isotope tracing on cancer ...

Therefore, to be able to understand how the metabolism of a tumor cell in its physiological environment differs from that of normal cells, these ...

metabolic diversity in human non-small cell lung cancer cells ...

Fig 3.2 Tracing metabolic flux using stable isotopes. In stable isotope tracing analysis, cells are cultured in medium containing 13C labeled ... In vivo analysis ...

Stable Isotope Tracing in vivo Reveals A Metabolic Bridge Linking ...

Here, we find that epithelial cells from germ-free mice harbor a loss of histone H4 acetylation across the genome except at promoter regions.

Physiological impact of in vivo stable isotope tracing on cancer ...

Cancer cells undergo diverse metabolic adaptations to meet the energetic demands imposed by dysregulated growth and proliferation. Assessing ...

Interrogating in vivo T-cell metabolism in mice using stable isotope ...

Ma, E. H. et al. Metabolic profiling using stable isotope tracing reveals distinct patterns of glucose utilization by physiologically activated CD8+ T cells.

Stable Isotope Tracing Analysis in Cancer Research - MDPI

Stable isotope tracing has emerged as a widely adopted tool for monitoring cancer metabolism both in vitro and in vivo. Advances in instrumentation and the ...

Stable isotope tracing to assess tumor metabolism in vivo

We have developed methods to use stable isotope-labeled nutrients (e.g., [C]glucose) to probe metabolic activity within intact tumors in vivo, in mice and ...

Metabolism – Cambridge Isotope Laboratories, Inc.

... metabolism research as tracers to quantify biochemical or metabolic reactions in vivo ... ➤ Stable Isotope Tracing in Cancer Metabolism · ➤ The Impact of Stable ...

Interrogating in vivo T-cell metabolism in mice using stable isotope ...

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