Structure and integrity of the purified RV VP6 and NoV GII.4 VLPs ...

Download scientific diagram | Structure and integrity of the purified RV VP6 and NoV GII.4 VLPs. The proteins were produced in a baculovirus-insect cell ...

Rotavirus VP6 Adjuvant Effect on Norovirus GII.4 Virus-Like Particle ...

Structure and integrity of the purified RV VP6 and NoV GII.4 VLPs. The proteins were produced in a baculovirus-insect cell system and purified ...

Norovirus VLPs and rotavirus VP6 protein as combined vaccine for ...

We have combined NoV GII-4 VLPs and human RV recombinant VP6 (rVP6) protein ... Norovirus GII-4, GII-12 and GI-3 VLPs production and purification. RNA ...

Structure and integrity of the proteins. Electron microscopy images of...

Electron microscopy images of RV VP6 nanotubes (a), trimeric VP6 (b), and NoV GII.4 VLPs (c). Protein products were examined by FEI Tecnai F12 electron ...

Full article: Rotavirus capsid VP6 protein acts as an adjuvant in vivo ...

Electron micrographs of the highly purified NoV GII.4 VLPs (A. Display full size. RV VP6 adjuvant effect on NoV type-specific serum antibodies. Groups of BALB ...

Rotavirus capsid VP6 tubular and spherical nanostructures act as ...

Structure and integrity of the proteins. Electron microscopy images of baculovirus–insect cell system-produced norovirus (NoV) GII.4-1999 virus-like particles ( ...

Combination of three virus-derived nanoparticles as a vaccine ...

To examine immunogenicity of EV-NoV-RV vaccine, BALB/c mice were immunized intramuscularly twice with 10 µg CVB1 VLPs, GII.4 VLPs and rVP6 ...

Rotavirus VP6 as an Adjuvant for Bivalent Norovirus Vaccine ... - MDPI

Electron micrographs of the highly purified plant-produced norovirus (NoV) (A) GI.4 VLPs, (B) GII.4-2006a VLPs, (C) recombinant RV VP6 protein ...

Simultaneous Immunization with Multivalent Norovirus VLPs ... - MDPI

NoV virus-like particles (VLPs) vaccine was investigated. Mice were immunized with NoV GI.3, GII.4 ... 3 VLPs + RV VP6 (the trivalent NoV-RV combination ...

Norovirus capsid and rotavirus VP6 protein for use as combined ...

... (GII-4 VLPs and dl VP2/VP6) Mix of VLPs). ... A comparison of methods for purification and concentration of norovirus GII-4, capsid virus-like particles ...

Research Article Rotavirus Recombinant VP6 Nanotubes ... - Trepo

integrity of RV VP6 ... highly purified VP6 nanotubes, GII.4 VLP, or LPS induced ... Figure 6: Internalization and activating/maturating effect of NoV GII.4 VLPs ...

Full article: Comparative immunogenicity in mice of rotavirus VP6 ...

RV capsid VP6 protein is highly conserved and the most abundant RV protein forming highly immunogenic oligomeric structures with multivalent antigen expression.

Simultaneous Immunization with Multivalent - ProQuest

We have comprehensively studied the immunogenicity of the trivalent NoV VLP and RV VP6 ... 4 and GII.4-2006 NoV VLPs. The technical ... Electron microscopy images ...

Trivalent Combination Vaccine Induces Broad Heterologous ... - PLOS

Cross-reactive IgG antibodies were also elicited against heterologous NoV VLPs not used for immunization (GII-4 NO, GII-12 and GI-1 VLPs) and to different RVs ...

Untitled - Istituto Superiore di Sanità

... VLPs and NoV GII-4 capsid VLPs were produced separately in Sf9 cells by ... Purity and integrity ... Both IgG1- and IgG2a-subtype serum antibodies against RV VP6.

Rotavirus Inner Capsid VP6 Acts as an Adjuvant in Formulations ...

containing RV VP6 nanostructures and norovirus (NoV) ... marrow-derived dendritic cells (BMDCs) pulsed with NoV GII.4 VLPs were employed as APCs as ...

Generation of Recombinant Rotaviruses Expressing Human ...

... RV and NoV diseases, a trivalent vaccine containing two types of NoV GII.4 VLPs (GII.4 NO-2010 and GII.4 SYD-2012) and oligomeric RV VP6 was ...

Deciphering structural variations within the norovirus capsid

Using cryo-electron microscopy, we determined the structure of different clinically relevant. GII.4 genotype virus like particles (VLPs), and an engineered ...

Significance of the Norovirus GII.4 Genotype as a Cause of ... - Trepo

GI.3, GII.4 VLPs and RV VP6 protein. All ... combination cannot confirm the native quaternary structure integrity of purified ... gradient-purified NoV GII-4 VLPs.

NOROVIRUS CAPSID AND ROTAVIRUS VP6 PROTEIN FOR USE ...

attributes of NV VLPs and RV VP6 protein which make ... Further, the GII-4 VLPs were purified with an ... GII-4 VLPs, rVP6 tubules, and dl VP2/VP6 VLPs were.