Template‐specific optimization of NGS genotyping pipelines reveals ...

Template-specific optimization of NGS genotyping pipelines reveals ...

We provide novel genotyping protocols for the three-spined stickleback MHC-I and -II genes, and evaluate the performance of popular NGS-genotyping pipelines.

We provide novel genotyping protocols for the three-spined stickleback MHC-I and -II genes, and evaluate the performance of popular NGS-genotyping pipelines.

Template-specific optimization of NGS genotyping pipelines reveals ...

Request PDF | Template-specific optimization of NGS genotyping pipelines reveals allele-specific variation in MHC gene expression | Using ...

Template‐specific optimization of NGS genotyping pipelines reveals ...

AbstractUsing high‐throughput sequencing for precise genotyping of multi‐locus gene families, such as the major histocompatibility complex (MHC), ...

DataONE Data Catalog

Template-specific optimization of NGS genotyping pipelines reveals allele-specific variation in MHC gene expression. Artemis Efstratiou, Arnaud Gaigher, Sven ...

Template-specific optimization of NGS genotyping pipelines reveals ...

Article "Template-specific optimization of NGS genotyping pipelines reveals allele-specific variation in MHC gene expression" Detailed information of the ...

Template-specific optimization of NGS genotyping pipelines reveals ...

Article: Template-specific optimization of NGS genotyping pipelines reveals allele-specific variation in MHC gene expression.

Reliable NGS genotyping of MHC class I and II genes ... - TechRxiv

... template-specific pipeline optimization for reliable genotyping of multi-locus gene families. ... Lastly, sibship-assisted cDNA genotyping of MHC-I revealed ...

Artemis Efstratiou - Advance

... NGS-genotyping pipelines and highlight the need for template-specific optimization for reliable multi-locus genotyping. Advance. | Powered by Authorea.com.

Optimization of Genotype by Sequencing data for phylogenetic ...

Given that both de novo and reference genome pipelines are often used to assemble next generation sequencing (NGS) data, and that several tree inference ...

Optimization of the genotyping‐by‐sequencing SNP calling for ...

A robust Genotyping-By-Sequencing (GBS) pipeline platform was examined to provide accurate discovery of Single Nucleotide Polymorphisms ...

Immunogenetic variation in the three-spined stickleback

... Template-specific optimization of NGS genotyping pipelines reveals allele-specific variation in MHC gene expression. Molecular Ecology ...

Breed-specific reference sequence optimized mapping accuracy of ...

... NGS reads of the sample ... Complete mitogenome reveals genetic divergence and phylogenetic relationships among Indian cattle (Bos indicus) breeds ...

Best practices for variant calling in clinical sequencing

Standard pipelines for NGS analysis. a Alignment and pre-processing of NGS data for an individual sample. Raw sequence data in FASTQ format ...

Genotyping-by-sequencing-based high-resolution mapping reveals ...

Through locus-specific marker enrichment and recombinant screening of ∼1,000 additional genotypes, we refined the originally postulated 5-mb veraison locus, ...

Streamlined whole-genome genotyping through NGS-enhanced ...

First, we perform TAIL-PCR using barcoded SP and AD primers (Figure 1A). After PCR, fragments selected from each sample are assigned specific barcodes. Second, ...

Browse Preprints - Authorea

... template-specific pipeline optimization for reliable genotyping of multi-locus gene families. ... Targeted NGS revealed that 15.07% (11/73) patients had ...

1 Good Laboratory Practice for Clinical Next-Generation ...

guidance for the establishment and optimization of clinical NGS informatics pipelines. ... To recalibrate quality scores, NGS data analysis pipelines, for example ...

Decoding the Mystery of Index Hopping in NGS - seqWell

The cause of this error is a phenomenon known as “index hopping,” which is when data from one sample “hops” to another index during multiplexed sequencing.

Guidelines for Validation of Next-Generation Sequencing–Based ...

At a specific depth of sequence coverage, the number of independent DNA template ... genome reveal new insights into the molecular diagnosis of mitochondrial DNA ...