Unaccounted uncertainty from qPCR efficiency estimates entails ...

The commonly used efficiency corrected Δ Δ C q and many other approaches to the analysis of qPCR data disregards the uncertainty of the ...

Unaccounted uncertainty from qPCR efficiency estimates entails uncontrolled ... BMC Bioinformatics. 2016 Apr 11:17:159. doi: 10.1186/s12859-016-0997-6.

Unaccounted uncertainty from qPCR efficiency estimates entails ...

Dive into the research topics of 'Unaccounted uncertainty from qPCR efficiency estimates entails uncontrolled false positive rates'. Together they form a unique ...

Unaccounted uncertainty from qPCR efficiency estimates entails ...

This article is in the 15th percentile (ranked 220,362nd) of the 300,920 tracked articles of a similar age in all journals and the 3rd percentile (ranked ...

Unaccounted uncertainty from qPCR efficiency estimates entails ...

Background Accurate adjustment for the amplification efficiency (AE) is an important part of real-time quantitative polymerase chain reaction (qPCR) ...

Unaccounted uncertainty from qPCR efficiency estimates entails ...

Unaccounted uncertainty from qPCR efficiency estimates entails uncontrolled false positive rates. Anders E. Bilgrau, Steffen Falgreen, Anders Petersen, ...

Unaccounted uncertainty from qPCR efficiency estimates entails ...

Unaccounted uncertainty from qPCR efficiency estimates entails uncontrolled false positive rates. BACKGROUND: Accurate adjustment for the amplification ...

How good is a PCR efficiency estimate: Recommendations for ...

Using a Monte Carlo approach, we find the uncertainty in the PCR efficiency estimation may be as large as 42.5% (95% CI) if standard curve with only one qPCR ...

Statistical uncertainty and its propagation in the analysis of qPCR data

Hence we approximate t by ... Unaccounted uncertainty from qPCR efficiency estimates entails uncontrolled false positive rates. Article. Full-text available.

Unaccounted uncertainty from qPCR efficiency estimates entails ...

Unaccounted uncertainty from qPCR efficiency estimates entails uncontrolled false positive rates-article.

Realizing the value in “non-standard” parts of the qPCR ... - NCBI

The displayed standard curve model includes Poisson-distributed reaction random sampling error and has intercept 36, amplification efficiency E ...

Table 2 DLBCL data: Method comparison for estimating the C q -value

This unaccounted uncertainty from PCR efficiency estimates entails uncontrolled false positive rates that are a significant problem in using qRT-PCR as a ...

Assessment of Mathematical Approaches for the Estimation and ...

Quantitative PCR is a molecular technique for DNA quantification that depends on reaction efficiency and the Ct value (“cycle threshold”).

PCR/qPCR Data Analysis - Sigma-Aldrich

... estimate amplification efficiency and target quantities in measured samples2,3 ... Error bars show the confidence interval of the difference between means.

Troubleshooting qPCR: Interpreting Amplification Curves

Troubleshooting abnormal amplification plots · Amplification efficiency is below 88% in one or both samples · Differences in efficiency are >5% ...

Evaluation of qPCR curve analysis methods for reliable biomarker ...

Unaccounted uncertainty from qPCR efficiency estimates entails uncontrolled false positive rates. Bilgrau AE, Falgreen S, Petersen A, Kjeldsen MK, Bødker JS ...

Understanding qPCR Efficiency and Why It Can Exceed 100%

This artifact can usually be avoided by using highly diluted samples. If inhibition occurs, concentrated samples should be excluded from the analysis when ...

AEBilgrau/effadj: Variance approximations of efficiency adjusted ...

git repository for the paper: # # # # Unaccounted uncertainty from qPCR efficiency estimates # # entails uncontrolled false positive rates # # # # Bilgrau ...

Comparing real-time quantitative polymerase chain reaction ...

New methods are used to compare seven qPCR analysis methods for their performance in estimating the quantification cycle (Cq) and amplification efficiency ...

qpcR: Modelling and Analysis of Real-Time PCR Data

Batch calculation of qPCR efficiency and other qPCR parameters. Description ... ## Individual efficiencies for error calculation. DAT1 <- pcrbatch(reps ...