Variability in RT|qPCR assay parameters indicates unreliable SARS ...

Variability in RT-qPCR assay parameters indicates unreliable SARS ...

Due to the coronavirus disease 2019 (COVID-19) pandemic, wastewater surveillance has become an important tool for monitoring the spread of ...

Variability in RT-qPCR assay parameters indicates ... - Collabovid

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Variability in RT-qPCR assay parameters indicates unreliable SARS ...

Variability in RT-qPCR assay parameters indicates unreliable SARS-CoV-2 RNA quantification for wastewater surveillance ... Authors: Aaron Bivins; Devrim Kaya ...

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In the present study we developed a sensitive RT-qPCR assay designed for the rapid, direct detection of Gamma and Delta variants of concern.

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Variability in RT-qPCR assay parameters indicates unreliable SARS-CoV-2 RNA quantification for wastewater surveillance. Water Res 203:117516 ...

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Variability in RT-qPCR assay parameters indicates unreliable SARS-CoV-2 RNA quan- · tification for wastewater surveillance. Water Res. 117516. Blanchard, P ...

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Variability in RT-qPCR assay parameters indicates unreliable SARS-CoV-2 RNA quantification for wastewater surveillance. Bivins, Aaron; Kaya ...

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Variability in RT-qPCR assay parameters indicates unreliable SARS-CoV-2 RNA quantification for wastewater surveillance. Water Res. 203 ...

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Variability in RT-qPCR assay parameters indicates unreliable SARS-CoV-2 RNA quantification for wastewater surveillance. Water Res. (2021) 203:117516. doi ...

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In addition to RT-qPCR inhibition susceptibility, the two assays showed significantly different SARS-CoV-2 concentration with FV exhibiting a ...

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Variability in RT-qPCR assay parameters indicates unreliable SARS-CoV-2 RNA quantification for wastewater surveillance. Water Res. 2021, 203 ...

(PDF) Inherent Bias of SARS-CoV-2 RNA Quantification for ...

We find that variation in these parameters and deviations from best practices as described in The Minimum Information for Publication of ...

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Variability in RT-qPCR assay parameters indicates unreliable SARS-CoV-2 RNA quantification for wastewater surveillance. Water Res. 2021; 203: 117516. 15. Kim ...

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For all RT-qPCR and RT-dPCR assays, SARS-CoV-2 RNA was only quantifiable at seeding values ≥2.32 × 104 GC/50 mL wastewater as shown in Table 4 . The US CDC N2 ...

Inherent bias of SARS-CoV-2 RNA quantification for wastewater

surveillance due to variable RT-qPCR assay parameters. Aaron Bivins1 ... The performance of one-step and two-step. RT-qPCR assays were compared in ...

What is a Cycle Threshold Value? - IDSA

In contrast, high Cts nearer to the assay's limit of detection (often ~40 cycles) indicate trace amounts of viral RNA in the tested sample.

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This reduced variability not only shows that the amount of human material present in NTS samples impacts on assay sensitivity, but also suggests that ...

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The assay was performed following the CDC protocol for SARS-CoV-2 detection using synthesized SARS-Cov-2 RNA as template, diluted into 105, 104, 103, 102, and ...