innovative CRISPR approaches for functional studies of long non ...

We discuss some of the novel variations to the classic CRISPR/Cas9 system that have been tailored and applied previously to study several aspects of lncRNAs ...

Long non-coding RNAs (lncRNAs) makeup a considerable part of the non-coding human genome and had been well-established as crucial players in ...

[PDF] Beyond classic editing: innovative CRISPR approaches for ...

Beyond classic editing: innovative CRISPR approaches for functional studies of long non-coding RNA · 16 Citations · 140 References.

CRISPR Methods Virtual Collection - Oxford Academic

Beyond classic editing: innovative CRISPR approaches for functional studies of long non-coding RNA. Dahlia A Awwad. Single-tube genotyping for small insertion ...

Functional knockout of long non-coding RNAs with genome editing

Various approaches are available, including RNA silencing, antisense oligos, and CRISPR-based genome editing. CRISPR-based genome editing is the ...

A CRISPR/Cas13-based approach demonstrates biological ... - Nature

However, CRISPR/Cas13 has not been used for functional studies of lncRNAs or mRNAs. Furthermore, it has not been applied in the context of ...

Targeting long non-coding RNAs in cancer therapy using CRISPR ...

The CRISPR-Cas9 system has been widely applied in functional genomics, gene therapy, and cancer research, offering a versatile platform for ...

Genetic Editing of Long Noncoding RNA Using CRISPR/Cas9 ...

Beyond classic editing: innovative CRISPR approaches for functional studies of long non-coding RNA · Biology, Medicine. Biology methods & protocols · 2019.

Full article: Interrogating lncRNA functions via CRISPR/Cas systems

Nevertheless, the majority of lncRNAs are low in abundance and poorly conserved, posing challenges to functional studies. The CRISPR/Cas system, an innovative ...

Designing libraries for pooled CRISPR functional screens of long ...

High-throughput functional screening methods, notably those based on pooled CRISPR-Cas perturbations, promise to unlock the biological ...

CRISPR-Based Approaches for the High-Throughput ... - MDPI

The recent development of CRISPR/Cas9 technology has opened unprecedented opportunities to uncover the genetic and functional characteristics of the non-coding ...

CRISPR/Cas9-mediated LINC00511 knockout strategies, increased ...

The growing detection of long noncoding RNAs (lncRNAs) required the application of functional approaches in order to provide absolutely ...

Techniques for investigating lncRNA transcript functions in ... - Nature

CRISPR-based approaches can be beneficial for the functional interrogation of lncRNAs [45], including those that are tissue-specific and/or ...

CRISPRi-based genome-scale identification of functional long ...

We designed a CRISPRi Non-Coding Library (CRiNCL), which targets 16,401 lncRNA genes each with 10 sgRNAs per TSS, and applied this pooled screening approach to ...

CRISPR links to long noncoding RNA function in mice: A practical ...

This is evident in biomedical research where the ease and low-cost of CRISPR editing has made it the preferred method of manipulating the mouse genome, ...

CRISPR-Based Approaches for the High-Throughput ... - MDPI

Bester entitled “CRISPR-based approaches for the high-throughput characterization of long non-coding RNAs” provides a good overview of the techniques, with in ...

Genomic Editing of Non-Coding RNA Genes with CRISPR/Cas9 ...

Most recently, a revolutionary gene editing technology known as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR- ...

Genetic Editing of Long Noncoding RNA Using CRISPR/Cas9 ...

Awwad DA (2019) Beyond classic editing: innovative CRISPR approaches for functional studies of long non-coding RNA. Biol Methods Protoc 4(1):bpz017.https ...

Different variations of CRISPR/Cas9 and their applications in lncRNA...

... CRISPR/Cas9 and their applications in lncRNA functional studies ... Beyond classic editing: Innovative CRISPR approaches for functional studies of long non-coding ...

Update on functional analysis of long non-coding RNAs in common ...

The first stage of research on lncRNAs was from 1980 to 2000, during which lncRNAs were first identified using traditional gene mapping methods, with H19 being ...