maxibor/pcrEfficiency
maxibor/pcrEfficiency: pcr efficiency predictor - GitHub
pcr efficiency predictor. Contribute to maxibor/pcrEfficiency development by creating an account on GitHub.
testing.py - maxibor/pcrEfficiency - GitHub
pcr efficiency predictor. Contribute to maxibor/pcrEfficiency development by creating an account on GitHub.
pcrEfficiency: a Web tool for PCR amplification efficiency prediction
pcrEfficiency provides an easy-to-use web interface allowing the prediction of PCR efficiencies prior to web lab experiments thus easing quantitative real-time ...
pcrEfficiency: a Web tool for PCR amplification efficiency prediction
pcrEfficiency provides an easy-to-use web interface allowing the prediction of PCR efficiencies prior to web lab experiments thus easing ...
Understanding qPCR Efficiency and Why It Can Exceed 100%
Typically, desired amplification efficiencies range from 90% to 110%. The theoretical maximum of 100% indicates that polymerase enzyme is working at maximum ...
How good is a PCR efficiency estimate
We propose recommendations for the precise estimation of PCR efficiency: (1) one robust standard curve with at least 3–4 qPCR replicates at each concentration ...
qPCR Efficiency Calculator | Thermo Fisher Scientific - US
This calculator gives the amplification efficiency of a qPCR reaction based on the slope of the standard curve.
Commonly used terms in PCR - QIAGEN
A slope of less than –3.322 (e.g., –3.8) indicates a PCR efficiency <1. Generally, most amplification reactions do not reach 100% efficiency due to experimental ...
BioInformatics - PCR Efficiency in real-time PCR - Gene-Quantification
Estimation via calibration dilution curve and slope calculation. real-time PCR efficiency: E = 10^ [–1/slope].
How can we have an amplification efficiency greater than 100%?
This method is alternative to standard curve of serial dilutions for calculation of PCR efficiency. Even if you apply the standard curve method, ...