Aptamer Regulated Transcription for In|vitro Sensing and Transduction

Designed Signaling Aptamers that Transduce Molecular ...

We have engineered aptamers that contain fluorescent reporters and that signal the presence of cognate ligands in solution.

Aptamer-based CRISPR-Cas powered diagnostics of diverse ...

Aptamers' excellent sensitivity, specificity, in vitro synthesis, base-pairing, labeling, modification, and programmability has made them an ...

Regulation of mRNA translation by a photoriboswitch - eLife

In particular, light-activated regulatory RNAs are needed for spatiotemporal control of gene expression. To fill this gap, we used in vitro ...

Synthetic logic circuits using RNA aptamer against T7 RNA ... - bioRxiv

Utilizing the property of this aptamer, we implemented synthetic logic circuits in an in vitro transcription setting. As a step to quickly ...

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Aptamer Regulated Transcription for In-vitro Sensing and Transduction. Unmet ... Currently, most protein biomarker detection is still done by conventional protein ...

Aptamer Modules as Sensors and Detectors - LIMES-Institut

Structure of an RNA Polymerase II-RNA Inhibitor Complex Elucidates Transcription Regulation by. Noncoding RNAs. Nat. Struct. Mol. Biol. 2006, 13, 44–48. 4 ...

Aptamer and Thiol Co‐Regulated Color‐Shifting Fluorophores via ...

Due to the programmability of this RNA sensor, the design method of target-competitive aptamers is standardized, making it universally ...

Selection and Biosensor Application of Aptamers for Small Molecules

We then discuss different kinds of small molecule aptamer-based sensors described in literature and their applications, ranging from detecting drinking water ...

Nucleic Acid Aptamer-Based Biosensors: A Review - MDPI

Aptamers, short strands of either DNA, RNA, or peptides, known for their exceptional specificity and high binding affinity to target molecules, ...

Dual-specificity RNA aptamers enable manipulation of target ...

Further, by coupling riboswitches or an inducible expression system to aptamers, we enabled inducible regulation of protein-specific O- ...

RNA Aptamers: From Basic Science Towards Therapy - SpringerLink

RNA aptamers have also been expressed in living cells. These aptamers, also called intramers, can be used to dissect intracellular signal transduction pathways.

Recent Progress in Nucleic Acid Aptamer-Based Biosensors and ...

Since aptamers exist in nature but can also be artificially isolated from pools of random nucleic acids through a process called in vitro selection, they can ...

challenges and advances in aptamer-based biosensors

In addition, the molecular sensors that use aptamers can be modular, and this trait may facilitate the development of multifunctional biosensors ...

Publications | Soh Lab - Stanford University

"DNA-controlled Protein Fluorescence: Design of aptamer-split peptide ... Sensor for Aptamer-based Therapeutic Drug Monitoring .” IEEE Journal of ...

Efficient delivery of a DNA aptamer-based biosensor into plant cells ...

This demonstration opens a new avenue to apply DNA aptamer sensors for functional studies of various targets, including metabolites, plant ...

http://wrap.warwick.ac.uk

pected, we performed in vitro transcription of systems theo- ... may allow the sensing and regulation ... regulation of transcription in eukaryotes. Cell ...

Intracellular gene transcription factor protein‐guided MRI by DNA ...

To better understand the effect of AP-1 TF on signal transduction in living tissue, we took an alternative approach of generating a double- ...

https://www.frontiersin.org/journals/pharmacology/articles/10.3389 ...

... vitro transcription reaction. The ... Effector-triggered transcription factor regulation of gene transcription ... Although the presence of the aptamer ...

Systematic bio-fabrication of aptamers and their applications in ...

Aptamers are obtained in vitro based on the systematic evolution of ligands by exponential enrichment (SELEX) technology. Usually, targets are ...

EP3551755A4 - Use of biological rna scaffolds with in vitro selection ...

... aptamer sensor. WO2024118806A1 * 2022-11-29 2024-06-06 The Johns Hopkins University Aptamer regulated transcription for in-vitro sensing and transduction.