Competitive polymerase chain reaction using an internal standard

Comparison of Two Quantitative Polymerase Chain Reaction ...

Five tenfold serially diluted external standards ranging from 1000,000 to 100 copies along with 4 ng of each unknown sample comprised the real-time PCR runs.

Gene Quantification & competitive RT-PCR

Other methods typically rely on end-point measurements, when often the reaction has gone beyond the exponential phase because of limiting reagents. To ...

Quantitative polymerase chain reaction analysis by deconvolution of ...

The qPCR is standardized by admixture of the amplicon product as an internal benchmark, and a computational algorithm is applied to determine ...

REVIEW Absolute quantification of mRNA using real-time reverse ...

1999). In competitive RT-PCR, the internal standard shares the same primer recognition and internal sequences with the primary target, leading to.

Quantitation of mRNA by the polymerase chain reaction. - PNAS

A method for the quantitation of specific mRNA species by the polymerase chain reaction (PCR) has been developed by using a synthetic RNA as an internal ...

Quantitation of HCV-replication using one-step competitive reverse ...

Quantitation of HCV-replication using one-step competitive reverse transcription-polymerase chain reaction and a solid phase, colorimetric detection method.

A quantitative description of the competitive polymerase chain reaction

QC-PCR is therefore similar to that in the standard PCR, with the minor ... fragments for use as internal standards in competitive PCR, PCR Meth-. &'c ...

Quantitation of Helicobacter pylori in dental plaque samples by ...

Several quantitative PCR assay methods have been reported. Competitive PCR (cPCR) is appropriate for the quantitation of small amounts of the target sequence ...

Smartphone-based chemiluminometric hybridization assays and ...

... PCR using a synthetic RNA internal standard, Anal. ... Tani, Quantitative method for specific nucleic acid sequences using competitive polymerase chain reaction ...

Range of Phytoplasma Concentrations in Various Plant Hosts as ...

For competitive polymerase chain reaction (PCR), an internal standard DNA ... The internal standard was calibrated using a quantified culture of ...

Detection and quantitation of genetically marked acute myeloid ...

Quantitation of residual blasts using a competitive polymerase chain reaction assay ... Quantitation of leukemic blasts was carried out by adding serial dilutions ...

PCR MIMICS: competitive DNA fragments for use as internal ...

To perform quantitative PCR, known quantities of PCR MIMICS are spiked into PCR amplification reactions containing the experimental cDNA samples. A visual or ...

Polymerase Chain Reaction–Based Method for Quantifying ...

Amplification of internal standard with use of the same primers produced a 602-bp band, easily separated from the Sry gene product. Competitive ...

Quantification of poliovirus in seawater and sewage by competitive ...

This method, using a cloned internal standard and specified primers in the PCR, may be applied to quantify other microorganisms in environmental samples.

Persistent DNA Contamination in Competitive RT-PCR Using cRNA ...

Accurate quantification of mRNA by competitive RT-PCR demands that the qual- ity of the cRNA internal standard be strictly controlled and that at least two ...

The Stress Kit - jstor

plification of cellular mRNA-derived cDNA with a dilution series of a competitor fragment (internal standard), using 1 ... using the polymerase chain reaction ( ...

Measurement of PTH/PTHrP receptor mRNA using a competitive ...

Measurement of PTH/PTHrP receptor mRNA using a competitive internal standard ... using a competitive internal standard'. Together ... Polymerase Chain Reaction ...

Quantitative polymerase chain reaction for the detection ...

This method is based on coamplification of an internal standard and a target DNA sequence with one set of primers. The internal standard was prepared using a ...

Real-time Polymerase Chain Reaction - Journal of American Science

Absolute quantitation, using competitive RT-PCR ... The Classic I and Classic II 18S Internal Standards by Ambion can be used with any vertebrate RNA sample.

Simultaneous Quantitation of Several mRNA Species by Calibrated ...

The amount of a specific mRNA can be determined by reverse transcription polymerase chain reaction (RT-PCR) coamplification of sample RNA ...