Evaluation of 13C isotopic tracers for metabolic flux analysis in ...

Figure 1 from Isotopically Nonstationary 13 C Metabolic Flux Analysis

Fig. 1. Overview of different MFA methodologies. The relative speed of metabolic and isotopic dynamics will influence the type of MFA study performed.

1 Isotopically Nonstationary 13C Metabolic Flux Analysis in Resting ...

Isotope assisted metabolic flux analysis (13C-MFA) is the current ... Evaluation of 13C isotopic tracers for metabolic flux analysis in ...

Isotopically Nonstationary 13C Metabolic Flux Analysis

The ability to quantitatively map intracellular carbon fluxes using isotope tracers and metabolic flux analysis (MFA) is critical for identifying pathway ...

13C tracer analysis suggests extensive recycling of endogenous ...

13C tracer analysis is increasingly used to monitor cellular metabolism in vivo and in intact cells, but data interpretation is still the ...

13c-labeled glucose - Sigma-Aldrich

A comprehensive metabolic profile of cultured astrocytes using isotopic transient metabolic flux analysis and C-labeled glucose. Ana I ...

Isotopic Tracer Methods of Monitoring Human Carbohydrate ...

While isotopes of hydrogen and oxygen exist and are commonly used in combined form in the exercise sciences for energy expenditure applications ...

A Primer to 13C Metabolic Flux Analysis - Wiley Online Library

It focuses on 13C MFA method that extends classical stoichiometric MFA by using intracellular isotope labeling data as an additional source of ...

13C Metabolic Flux Analysis - Institute of Molecular Systems Biology

Since reaction rates are not di per se detectable, stable isotopic tracers are used to infer them form the propagation of 13C over time (in dynamic experiments) ...

Publishing 13C metabolic flux analysis studies: a review and future ...

(13)C-Metabolic flux analysis ((13)C-MFA) is a powerful model-based analysis technique for determining intracellular metabolic fluxes in living cells.

Visual workflows for 13 C-metabolic flux analysis - Oxford Academic

Motivation: The precise quantification of intracellular metabolic flow rates is of fundamental importance in bio(techno)logy and medical ...

High Quality 13C metabolic flux analysis using GC-MS

(2009), Evaluation of 13C isotopic tracers for metabolic flux analysis in mammalian cells. Journal of Biotechnology, 144,. 167-174. 12. Quek, L.-E., Wittmann ...

A roadmap for interpreting 13C metabolite labeling patterns from cells

Optimization of 13C isotopic tracers for metabolic flux analysis in ... data evaluation in 13C flux analysis: mass isotope correction, · data consistency ...

C-Fingerprinting and Metabolic Flux Analysis of ... - OSTI.GOV

13C-isotopic tracers have been an essential tool in determining metabolic fluxes, in vivo reaction rates, within the cell central metabolism. This 13C- ...

Frontiers | Tandem Mass Spectrometry for 13C Metabolic Flux Analysis

In 13C-MFA, labeling experiment are performed by introducing a 13C labeled substrate (the tracer), followed by measurement of 13C labeling ...

One‐shot 13C15N‐metabolic flux analysis for simultaneous ...

Recently, multiple stable isotopic tracers have been used to measure C and N enrichments and to derive insights into N assimilation in ...

Metabolic flux analysis: a comprehensive review on sample ...

In the last decade, the application of stable isotopes became extremely important to determine and integrate in vivo measurements of metabolic ...

MIT Open Access Articles A roadmap for interpreting 13C metabolite ...

Selection of tracers for 13C-metabolic flux analysis using elementary ... 13C metabolic flux analysis: optimal design of isotopic labeling experiments.

13C isotope-based metabolic flux analysis revealing cellular ... - OUCI

13C isotope-based metabolic flux analysis revealing cellular landscape of glucose metabolism in human liver cells exposed to perfluorooctanoic acid · List of ...

FreeFlux: A Python Package for Time-Efficient Isotopically ...

C metabolic flux analysis (13C MFA) aims to quantify the intracellular metabolic activity represented by the velocity of interconnecting ...

Measuring Metabolic Flux - The Animation Lab

Estimated fluxes are highly dependent on the specific choice of isotopic tracers. Optimal tracers are identified via in silico simulation. There can be single, ...