Harnessing DSB repair to promote efficient homology|dependent and

CRISPR 101: Homology Directed Repair - Addgene Blog

HDR uses homologous sequence as a template for repair and is thus the more accurate mechanism for DSB repair. The process is generally error- ...

Novel gene editing offers efficient route to therapies - AstraZeneca

Harnessing DSB repair to promote efficient homology-dependent and -independent prime editing. Nature Communications 2022; March 24th. Veeva ...

Genome Editing of Mammalian Cells Through RNA Transcript ...

Double-stranded break (DSB) repair of eukaryotic DNA is mainly accomplished by nonhomologous end joining and homologous recombination (HR).

CRISPR/Cas9 and Agrobacterium tumefaciens virulence proteins ...

Further optimization of the CvDT process with a donor linker (CvDTL) achieved a remarkable increase in the efficiency of HDR-mediated genome editing.

Inhibition of nonhomologous end joining to increase the specificity of ...

Non- homologous end joining (NHEJ) and homologous recombination are two major double-strand break repair pathways. SCR7, a DNA ligase IV inhi- bitor, was ...

Our approach to gene therapy | AstraZeneca

Harnessing DSB repair to promote efficient homology-dependent and -independent prime editing. Nature Communications 2022; March 24th. 5. Wimberger S, Akrap N ...

Strategies to improve homology-based repair outcomes following ...

DSBs are the most cytotoxic form of DNA damage and as a result eukaryotic cells have developed a sophisticated DNA damage response (DDR), ...

Improved nuclease-based prime editing by DNA repair modulation ...

In this work, we introduce two strategies to enhance PEn precision and efficiency. First, we apply a small molecule approach, selectively ...

Modulating DNA Repair Pathways to Improve Precision Genome ...

The DSB is repaired by endogenous DNA repair pathways, either nonhomologous end joining (NHEJ) or homology directed repair (HDR). The ...

Utilizing DSB repair pathways for gene editing. The processing of ...

... Homologous Recombination and DNA Repair | ResearchGate, the professional network ... Scientists have explored various approaches to enhance HDR efficiency.

Harnessing CRISPR-Cas9 for Genome Editing in Streptococcus ...

After a precise double-stranded break (DSB) is introduced, the cells' DNA repair mechanism of homology-directed repair (HDR) is exploited to select successful ...

Improving homology‐directed repair by small molecule agents for ...

Small molecules that effectively inhibit DSB repair are toxic to ... (1999). XRCC3 promotes homology- directed repair of DNA damage in mammalian cells.

Maximizing the Efficacy of CRISPR/Cas Homology-Directed Repair ...

In mammalian cells, the CRISPR/Cas-generated DSBs can be repaired by either template-free error-prone end joining (e.g., non-homologous end joining/ ...

Improving homology‐directed repair by small molecule agents for ...

Beyond plasmids, HR-mediated DSB repair via donor DNA is an essential requirement for precise genome editing such as the insertion of pathways ...

pCMV-PE2 Citations - Addgene

Harnessing DSB repair to promote efficient homology-dependent and -independent prime editing. ... homology-directed-repair-biased CRISPR genome editing.

Harnessing DNA Double-Strand Break Repair for Cancer Treatment

Compared to healthy cells, cancer cells have a higher. DSB burden due to oncogene-induced replication stress and acquired defects in DNA damage ...

Improved nuclease-based prime editing by DNA repair modulation ...

Harnessing DSB repair to promote efficient homology-dependent and ... Efficient high-precision homology-directed repair-dependent genome editing by ...

Current Strategies for Increasing Knock-In Efficiency in CRISPR ...

Major steps in non-homologous end joining (NHEJ) and Homology-directed repair (HDR). NHEJ is initiated by binding of Ku to the DSB ends. This is followed by ...

Efficient precise integration of large DNA sequences with 3 - PNAS

By comparison, the homology-directed repair (HDR) at the DSB site allows for precise knock-in (KI) of genes using exogenous donors with homology ...

strategies to enhance the frequency of homology-directed repair ...

Non-homologous end joining (NHEJ), and to a lesser extent, the error-free pathway known as homology-directed repair (HDR) are cellular mechanisms for ...