High|speed volumetric two|photon fluorescence imaging of ...

High-speed two-color scanning volumetric laser-induced fluorescence

PDF | Many problems in fluid mechanics require single-shot 3D measurements of fluid flows, but are limited by available techniques.

Two-Photon Laser Scanning Stereomicroscopy for Fast Volumetric ...

High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics. Jiang Lan Fan, Jose A. Rivera, Wei Sun, John Peterson, Henry Haeberle, Sam ...

Frontiers | Three-photon excited fluorescence imaging in neuroscience

High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics. Nat. Commun. 11, 1–12. doi: 10.1038/s41467-020-19851-1.

Axial gradient excitation accelerates volumetric imaging of two ...

We used Grad-TPM to capture the 3D process of macrophages phagocytizing fluorescent beads at a speed of 6 volume/min [Figs. 3(b)–3(d) and Visualization 3 ]. We ...

High-speed volumetric two-photon imaging of deep brain areas

Two-photon microscopy has revolutionized the ability to image neural activity in the brain of a living animal. Compared to single-photon ...

Two-Photon Fluorescence Microscopy - Newport

... high NA microscope objective. This combination produces a small focal volume where the intensity is high enough to drive the 2PF process. The NIR ...

High-speed, two-photon scanning microscope | MIT

ted fluorescence. Assuming a typical two-photon excitation volume of 0.1 fl and a fluorophore concen- tration of 10 ␮M, approximately 70 photons per pixel.

Multiphoton microscopy - Class 5 Photonics

... high power lasers for Multiphoton Microscopy ... Laser for 2-photon high-speed volumetric imaging · White Dwarf 3p. Laser for 3 ...

Two-photon excitation microscopy: Why two is better than one

This means infrared laser light has enough power to excite fluorophores up to around 1 mm in living tissues. In comparison, single photon confocal microscopy ...

Speed scaling in multiphoton fluorescence microscopy

High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics. Nat. Commun. 11, 6020 (2020). 59. Tan, X. J. et al. Volumetric two-photon ...

Publications - Guanghan Meng's website

& Ji, N. "High-throughput synapse-resolving two-photon fluorescence microendoscopy for deep-brain volumetric imaging in vivo". Elife (2019); Broussard, G. J. ...

Multiphoton fluorescence microscopy for in vivo imaging - Cell Press

reach, which will immediately impact high-speed imaging, such as ... Rapid volumetric imaging with. Bessel-Beam three-photon microscopy.

Extended two-photon microscopy in live samples with Bessel beams

... imaging speed in volume samples and ease of use. For this reason, we recently presented a simple and retrofittable modification to the two- ...

What Is Two-Photon Microscopy?

... (volumetric data), but at greater depths than conventional confocal microscopy. Two-photon lasers: the need for power and speed. Compared to ...

High-throughput synapse-resolving two-photon fluorescence ...

We applied this approach to the volumetric anatomical imaging of dendritic spines and axonal boutons in the mouse hippocampus, and functional imaging of ...

Fluorescent Probes for Two-Photon Microscopy—Note 1.5 - US

Defining Characteristics of Two-Photon Excitation · Spatial confinement of fluorescence to a very small volume (~0.1 μm3) defined by the focused excitation light ...

High-throughput volumetric imaging of neural dynamics in vivo

However, conventional two-photon fluorescence microscopy has to perform ... As a result, Bessel focus scanning technology enables high-speed volumetric imaging ...

Bessel Focus Scanning Two-Photon Microscopy and Optical ...

High-Speed Neural Imaging with Synaptic Resolution: Bessel Focus Scanning ... two-photon fluorescence microendoscopy for deep-brain volumetric imaging in vivo.

Multiphoton Microscopy - Nikon's MicroscopyU

... high laser power is required to generate significant two-photon-excited fluorescence. ... excitation volume in two-photon excitation microscopy is an advantage.

Initial studies of two-photon line excitation array detection ...

... two-photon fluorescence microscope is essential in volumetric brain imaging. ... imaging frame rate or a small-scale imaging technique with fast imaging speed.