Metabolic Flux Analysis and In Vivo Isotope Tracing

Metabolic Flux – Cambridge Isotope Laboratories, Inc.

These methods can translate also to the in vivo setting, with mice or ... C-MFA or 13C metabolic flux analysis is a stable isotope-assisted technique ...

Metabolic profiling of leukemic cells through isotope tracing in ...

This project will leverage cutting-edge metabolomics and isotopic labeling-based flux analysis ... vivo metabolism altered by leukemia? do cancer cells ...

Stable isotope tracing to assess tumor metabolism in vivo

High-resolution (13)C metabolic flux analysis. Nat. Protoc.14, 2856–2877 (2019). Yuan, M. et al. Ex vivo and in vivo ...

[PDF] Metabolic Flux Analysis—Linking Isotope Labeling and ...

13C tracer experiments and metabolite balancing for metabolic flux analysis ... vivo flux analysis was utilized to analyze metabolic modulation are reviewed.

13C tracer analysis suggests extensive recycling of endogenous ...

C tracer analysis is increasingly used to monitor cellular metabolism in vivo ... Stable isotope tracing has been used to probe specific metabolic ...

Tracing metabolic flux through time and space with isotope labeling ...

Young, INCA: a computational platform for isotopically non-stationary metabolic flux analysis, Bioinformatics, № 30, с. 1333

Isotope Tracing and 13C-Butyrate Metabolic Flux Analysis In Vivo (A)...

Schematic diagrams of 13C-butyrate tracing experiments in mice. (B–E) Abundance of isotopic labeling TCA cycle metabolites and SCFA in cecum (B), colon (C), ...

Toward metabolome-based 13 C flux analysis: a universal tool for ...

For most biological questions, stable isotope tracers must be administered and trackedto effectively determine metabolic fluxes by means of ...

Stable Isotope Tracing in vivo Reveals A Metabolic Bridge Linking ...

One of the more intriguing findings from the in vivo isotope tracing analysis ... flux from the microbiota to host fatty acid metabolism.

Ex vivo and in vivo stable isotope labelling of central carbon ...

13CFLUX2--high-performance software suite for (13)C-metabolic flux analysis. Bioinformatics29, 143–145 (2013). Huang, X. et al. X13CMS: global tracking of ...

Stable isotope-labeling studies in metabolomics

Recent efforts to incorporate isotopically nonstationary 13C metabolic flux analysis allow the study of incorporation of isotopes into metabolites in a system ...

In vivo isotope tracing reveals a requirement for the electron ...

Our data indicate that such labeling patterns can involve tumor cell–autonomous oxidative metabolism in the mitochondria and can be supported by ...

(PDF) Metabolic Flux Analysis-Linking Isotope Labeling and ...

The conceptual idea of MFA is that in tracer experiments the isotope labeling patterns of intracellular metabolites are determined by the fluxes ...

Quantitative Fluxomics of Circulating Metabolites

Here we develop isotope tracing, mass spectrometry, and mathematical analysis methods to determine ... 13C metabolic flux analysis. Metab. Eng. 3, 195–206 ...

Isotope-Assisted Metabolic Flux Analysis: A Powerful Technique to ...

... vivo. Just as with in vitro studies, the in vivo study tracer must be chosen to specifically represent flux through the tissue of interest ...

Systems Biology Approaches for Engineering Metabolism Using ...

The ML-Flux analysis frameworks is computationally light and deployed as a webtool. Thus, ML-assisted isotope tracing is a promising step ...

Quantitative Metabolic Flux Analysis Based on Isotope Labeling

Isotope-based MFA, short 13 C-MFA, is the most powerful tool for quantifying metabolic fluxes in vivo with highest possible accuracy.

Isotope Labeling in Metabolomics and Fluxomics, Charles Evans

Understand the difference between normal metabolomics and a flux experiment; how to perform an experiment using a stable isotope.

Global 13C tracing and metabolic flux analysis of intact human liver ...

... metabolism in intact human liver tissue ex vivo using global 13C tracing, non-targeted mass spectrometry and model-based metabolic flux analysis. Isotope ...

Global 13C tracing and metabolic flux analysis of intact human liver ...

Isotope tracing ex vivo allows measuring human liver metabolism with great depth and resolution in an experimentally tractable system. PUBLICATIONS. Global ...