Quantification of hepatitis C virus RNA by competitive amplification ...

Clinical characterization of a competitive PCR assay for quantitative ...

Rational clinical application of quantitative assessments of hepatitis C virus (HCV) RNA depends on an understanding of factors affecting the assay and its ...

Development of a sensitive RT-PCR method for amplifying and ...

The optimized method is able to successfully, accurately, and reproducibly amplify near full-length genotype 1 HCV RNA containing a wide range ...

HEPATITIS C VIRUS SUPPLEMENTAL PCR, QUANTITATIVE

The HCV RNA PCR procedure utilizes a real-time polymerase chain reaction test from Abbott Molecular. The amplification target is a conserved region of the HCV ...

Comparison of a competitive combined reverse ... - ASM Journals

Hepatitis C virus (HCV) is a positive-strand RNA virus ... Quantification of hepatitis B virus DNA by competitive amplification and ... hepatitis C. Lancet ...

Hepatitis C Virus RNA Functionally Sequesters miR-122 - Cell Press

We describe a quantitative mathematical model of HCV-induced miR-122 sequestration and propose that such miR-122 inhibition by HCV RNA may result in global de- ...

Biological dynamics of viral load in hemodialysis patients with ...

In the group with persistent viremia, the mean difference between maximum and minimum values of HCV-RNA observed in each individual patient was 2.09 ± 0.7 ...

Qualitative multiplex RT-PCR for simultaneous detection of hepatitis ...

Extraction and amplification of HCV and HIV-1 RNA from plasma samples were performed in a single reaction, and amplified genomes were detected with specific ...

Comparison of HCV RNA Levels by Branched DNA Probe Assay ...

HCV RNA was grouped into four genotypes by PCR with type-specific primers,and HCV RNA level was measured by branched DNA probeassay and by competitive PCR. HCV ...

Recombinase polymerase amplification as a promising tool in ...

Detection of HCV RNA level is an important factor in antiviral regimens especially for determination of SVR. RPA combines the advantages of serologic and ...

Quantitation of Hepatitis C Virus RNA | Annals of Internal Medicine

TO THE EDITOR: Gretch and colleagues [1] compared two quantitative hepatitis C virus (HCV) RNA assays: the branched DNA (bDNA) assay, commonly ...

Hepatitis C Virus RNA Strands Detection in Peripheral Blood ...

Conclusion: PBMCs PCR is a valid diagnostic test that can diagnose intracellular HCV when SRT-PCR is negative. Antisense and sense strands are ...

Hepatitis C – Laboratory case definition

3.1.2 HCV quantitative RNA detection. HCV RNA can be quantified by means of target amplification techniques (competitive PCR or real-time PCR) or signal ...

Relationship of genotype to level of hepatitis C viraemia determined ...

The level of HCV RNA (logarithmic transformed copy numbers per 50 μl of serum) was significantly higher in subjects of genotype II than in those of genotypes ...

Assessment of Hepatitis C Virus RNA Levels by Quantitative ... - jstor

Assessment of Hepatitis C Virus RNA Levels by Quantitative Competitive. RNA Polymerase Chain Reaction: High-Titer Viremia Correlates with Advanced Stage of ...

Rapid detection of hepatitis C virus using recombinase polymerase ...

Current PCR-based HCV RNA assays are restricted to well-resourced central laboratories, and there remains a prevailing clinical need for ...

A method for rapid detection and genotype identification of hepatitis ...

Total RNA extracts of HCV genotypes 1–6 were subjected to isothermal amplification. Electrophoretic analysis of reaction products demonstrated positive ...

The importance of HCV RNA measurement for tailoring treatment ...

Several virological tools including qualitative and quantitative HCV RNA assays for detection and quantification of HCV RNA are commercially available. We ...

Molecular Testing in the Diagnosis and Management of Hepatitis C ...

The HCV viral load assay from Biomerieux utilizes nucleic acid sequence–based amplification technology to amplify target RNA. Nucleic acid ...

Lyophilized standards for the calibration of real time PCR assay for ...

semiquantitative determination of hepatitis C virus. genome ... internationaI standard for hepatitis C virus RNA nucleic. acid amplification technology assays．

Stability of standard curves of hepatitis C virus transcripts used for ...

RNA was extracted from each point of the curve and amplified by competitive RT-PCR, using a fluorimetric detection scheme based on the use of separate, specific ...