Small Interfering RNAs Mediate Sequence|Independent Gene ...

Sequence, chemical, and structural variation of small interfering ...

Sequence, chemical, and structural variation of small interfering RNAs and short hairpin RNAs and the effect on mammalian gene silencing. · 474 Citations · 134 ...

siRNA Design Software for a Target Gene-Specific RNA ... - Frontiers

RNA interference (RNAi) is a mechanism through which small interfering RNA (siRNA) induces sequence-specific posttranscriptional gene silencing.

Viral Small Interfering RNAs Target Host Genes to Mediate Disease ...

Small RNA sequencing and 5′ RACE analyses confirmed that this 22-nt sequence was targeted for mRNA cleavage by Y-Sat-derived siRNAs. Transformation of tobacco ...

Comprehensive Review of Small Interfering RNAs (siRNAs) | IJN

Short nucleotide sequences are synthesized exogenously to create siRNA, which triggers RNA interference (RNAi) in cells and silences target gene ...

Gene Silencing Mediated by Small Interfering RNAs in Mammalian ...

... RNA interference (RNAi) is a post-transcriptional process in which double-stranded short interfering RNA (siRNA) triggers a sequence-specific suppression of ...

siRNA - Applications - Horizon Discovery

This activated protein and nucleic acid complex can then elicit gene silencing by binding, through perfect complementarity, to a single target mRNA sequence, ...

Functional anatomy of siRNAs for mediating efficient RNAi in ...

Duplexes of 21–23 nucleotide (nt) RNAs are the sequence‐specific mediators of RNA interference (RNAi) and post‐transcriptional gene silencing (PTGS).

Small-Interfering RNA (siRNA)-Based Functional Micro

An asymmetric dsRNA with 21 nucleotides of antisense strand and a shorter sense strand showed reduced nonspecific gene silencing without loss of ...

siRNA-Mediated Gene Silencing in Mammalian Cells - US

IntroductionSmall Interfering RNAs (siRNAs) are short double-stranded RNA molecules that can target and degrade complementary mRNAs via a cellular process ...

Short Interfering RNA-Mediated Inhibition of Herpes Simplex Virus ...

RNA interference (RNAi) is an antiviral mechanism that is activated when double-stranded RNA is cleaved into fragments, called short interfering RNA (siRNA) ...

Small interfering RNA (siRNA) - McManus Lab

siRNAs hold nearly limitless potential for use as a tool, which has led to their rise as the most widely used gene-silencing approach. To date, ...

Application of Small Interfering RNAs to Gastrointestinal Diseases

RNA interference (RNAi) is a naturally occurring cellular mechanism wherein foreign double-stranded RNA introduced into the cell induces posttranscriptional ...

Exogenous siRNA Mediates Sequence-Independent Gene ...

siRNA has been used to suppress host, viral, and exogenous genes. After determining that siRNA suppressed viral infection in a sequence- ...

Thermodynamic Control of Small RNA-Mediated Gene ... - Frontiers

In RNAi, siRNA loaded onto the RNA-induced silencing complex (RISC) downreugulates target gene expression by cleaving mRNA whose sequence is perfectly ...

RNA interference: learning gene knock-down from cell physiology

RNAi is mediated by the RNA-induced silencing complex (RISC) which, guided by siRNA, recognizes mRNA containing a sequence homologous to the ...

Sequence, Chemical, and Structural Variation of Small Interfering ...

For RT-. siRNA-MEDIATED GENE SILENCING. 85. Page 4. PCR analysis of hairpin RNA-mediated silencing, 20,000. A549 cells were seeded per well in 48-well plates ...

RNA silencing - Wikipedia

RNA silencing or RNA interference refers to a family of gene silencing effects by which gene expression is negatively regulated by non-coding RNAs such as ...

Relative gene-silencing efficiencies of small interfering RNAs ...

Abstract. Short interfering RNAs (siRNAs) directed against different regions of genes display marked variation in their potency in mediating mRNA degradati.

Endogenous activated small interfering RNAs in virus‐infected ...

Small interfering RNAs (siRNAs) are main actors in the regulation of gene expression by RNA silencing. The biogenesis and function of siRNAs ...

RNA interference is mediated by 21- and 22-nucleotide RNAs

Double-stranded RNA (dsRNA) induces sequence-specific posttranscriptional gene silencing in many organisms by a process known as RNA interference (RNAi).