Evaluation of 13C isotopic tracers for metabolic flux analysis in ...

Understanding metabolism with flux analysis

Evaluation of 13C isotopic tracers for metabolic flux analysis in mammalian cells. J. Biotechnol. 144, 167–174. http://dx.doi.org/10.1016/j ...

Improved 13 C metabolic flux analysis in Escherichia coli ...

Abstract . Gas chromatography–tandem mass spectrometry with electron ionization (GC–EI–MS/MS) provides rich information on stable-isotope labeling for 13C-

Interpreting metabolic complexity via isotope-assisted metabolic flux ...

Evaluation of 13C isotopic tracers for metabolic flux analysis in mammalian cells. J. Biotechnol. 2009; 144:167-174. Crossref · Scopus (231).

Isotopically nonstationary metabolic flux analysis of plants: recent ...

By introducing stable isotopic tracers and employing mathematical models, MFA can quantify the rates of metabolic reactions through biochemical ...

Stable Isotopes for Tracing Mammalian-Cell Metabolism in vivo - Lirias

Keywords: in vivo metabolism; stable-isotope tracers; tracer analysis; metabolic models ... (2009) Evaluation of 13C Isotopic Tracers for Metabolic Flux Analysis ...

Investigation of useful carbon tracers for 13C-metabolic flux analysis ...

The 13C-MFA experiments require an optimal design since the precision or confidence intervals of the estimated flux levels depends on factors such as the ...

Proposing a validation scheme for 13C metabolite tracer studies in ...

13C metabolite tracer and metabolic flux analyses require upfront experimental planning and validation tools. Here, we present a validation ...

Metabolic Flux – Cambridge Isotope Laboratories, Inc.

Isotope tracers are essential tools for monitoring metabolic pathway activity, ie flux. To quantitate flux in central carbon metabolism in cultured mammalian ...

Bayesian 13C-Metabolic Flux Analysis of Parallel Tracer ... - MDPI

One-µL aliquots of sample solution were injected in splitless or split mode depending on peak intensity (duplicate measurements). The mass spectrometer was ...

Validation-based model selection for 13C metabolic flux analysis ...

Accurate measurements of metabolic fluxes in living cells are central to metabolism research and metabolic engineering.

Metabolic networks in motion: 13 C‐based flux analysis - EMBO Press

Methods for experimental determination of metabolic fluxes differ fundamentally from component concentration measurements; that is, intracellular reaction rates ...

Applications of Stable Isotope Labeling in Metabolic Flux Analysis

Stable isotope labeling is a sophisticated technique, prominently recognized in the realm of metabolic flux analysis (MFA), that allows researchers to map the ...

Isotopic Tracer Methods of Monitoring Human Carbohydrate ...

... evaluating studies using stable isotopes to assess carbohydrate metabolism ... 13C isotopic tracers for metabolic flux analysis in mammalian cells. Journal ...

Ex vivo and in vivo stable isotope labelling of central carbon ...

Evaluation of 13C isotopic tracers for metabolic flux analysis in mammalian cells. J. Biotechnol.144, 167–174 (2009). Nissim, I. et al. Effects of a ...

Investigation of useful carbon tracers for 13 C-metabolic flux ... - Scite

glucose for 13C-metabolic flux analysis (13C-MFA) of Escherichia coli are investigated using a computer simulation of the stable isotope labeling experiment ...

Glucose-13c | Sigma-Aldrich

... isotopic transient metabolic flux analysis and C ... Here, we developed an efficient 13C-tracer dynamic metabolic flux analysis ...

Metabolic flux analysis: a comprehensive review on sample ...

In the last decade, the application of stable isotopes became extremely important to determine and integrate in vivo measurements of metabolic reactions in ...

Multi-objective experimental design for 13C-based metabolic flux ...

Evaluation of 13C isotopic tracers for metabolic flux analysis in mammalian cells. J. Biotech- nol. 144 (3), 167 – 174. Miettinen, K., 1999 ...

Adapting isotopic tracer and metabolic flux analysis - OSTI.GOV

Bold text indicates studies highlighted in this review. 136. 137. C1-Specific. Technique. Species. C1 Metabolism Insights. Reference.

Accurate Assessment of Amino Acid Mass Isotopomer Distributions ...

Metabolic flux analysis based on stable-isotope labeling experiments and analysis of mass isotopomer distributions (MID) of cellular ...