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Impact of CO2/HCO3– Availability on Anaplerotic Flux in Pyruvate ...


Process Design for the Biocatalysis of Value-Added Chemicals from ...

system during the initial growth phase did not significantly impact the CO2 ... Expression of anaplerotic enzyme pyruvate carboxylase improves recombinant protein ...

Tandem-Mass-Spectrometry-Driven Investigation of the Anaplerotic ...

in CO2 for flux inference. The biosynthesis of fatty acids as well as ... CO2/HCO3 levels on metabolism and regulation in Corynebacterium glutamicum.

Heterotrophic fixation of inorganic carbon - BG

flux from concurrent anaplerotic CO2 fixation. As a consequence, there are ... on the relationship between heterotrophic CO2 fixation and the availability of ...

Pyruvate in the Correction of Intracellular Acidosis: A Metabolic ...

Exogenous lactate (Lac) as an alternative of natural buffer, bicarbonate, consumes intracellular protons on an equimolar basis, regenerating ...

C allows a direct measure of flux through a single enzyme-catalyzed ...

[1-13C1]pyruvate in vivo should be sensitive to the availability of ... CO2, HCO3. ⫺, pyruvate C1, lactate C1, alanine C1, and pyruvate ...

Phosphoenolpyruvate Carboxykinase as the Sole Anaplerotic ...

The PEPCK reaction produces one ATP per carboxylation event, whereas the original route through pyruvate kinase and pyruvate carboxylase is ATP neutral. This ...

The PEP-pyruvate-oxaloacetate node - Oxford Academic

1997). In contrast to phosphoenolpyruvate carboxylase, PEPCK uses CO2 rather than HCO3. −, and has a specific binding site for CO2, which is ...

Activation and Inhibition of Pyruvate Carboxylase from Rhizobium etli

1.1) is an essential regulatory, anaplerotic enzyme which catalyzes the MgATP- dependent carboxylation of pyruvate by HCO3. – to form oxaloacetate, MgADP and Pi ...

Meeting the Metabolic Requirements of Cell Proliferation

Glucose is metabolized via glycolysis to pyruvate, which can be oxidatively metabolized to CO2 in the tricarboxylic acid. (TCA) cycle to generate large amounts ...

Both Forward and Reverse TCA Cycles Operate in Green Sulfur ...

Moreover, CO2 is essential for assimilat- ing acetate and pyruvate through the CO2-anaplerotic pathway ... pyruvate (acetate or pyruvate ⫹ HCO3.

Microfluidic single-cell scale-down bioreactors: A proof-of-concept ...

... the effects of ... Impact of CO2/HCO3-Availability on Anaplerotic Flux in Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum Strains.

Hyperpolarized Pyruvate (13C) Injection - Cancer Imaging Program

Effect of Fatty Liver on TCA Cycle Flux and the Pentose Phosphate Pathway ... The [13C]carbon dioxide released is subsequently interconverted with [13C] ...

PhD thesis JB - Universität Stuttgart

considerations such as flux analysis, especially under different CO2/HCO3 ... • Determination of the impact of oscillating CO2/HCO3. – conditions on l ...

The PEP-pyruvate-oxaloacetate node - WUR eDepot

The carboxyphosphate is then cleaved into hydrogen phosphate and the—compared to bicarbonate—more reactive CO2. Finally, the CO2 is fixed on the.

Hyperpolarized ¹³C Allows a Direct Measure of Flux through a Single ...

[l-13Ci]pyruvate in vivo should be sensitive to the availability of ... C02, HCO3, pyruvate C1, lactated, alanine C1, and pyruvate hydrate C1. The.

Intramolecular carbon isotope signals reflect metabolite allocation in ...

In illuminated leaves of Xanthium strumarium, flux from pyruvate to PEP is very small at ~0.05% of net CO2 assimilation (Tcherkez et al., 2011). In ...

potential of anaplerotic triheptanoin for the treatment of long-chain ...

(240) reported that increased anaplerotic pyruvate flux can be used for gluconeogenesis in rat livers. ... Effects of extracellular pH, CO2, and HCO3- on ...

Pyruvate Formate-Lyase Enables Efficient Growth of Escherichia coli ...

Pyruvate formate-lyase (PFL) is a ubiquitous enzyme that supports increased ATP yield during sugar fermentation. While the PFL reaction is ...

Three examples of anaplerotic reactions include - Quora

1. **Pyruvate Carboxylation:** - Reaction: Pyruvate + CO2 + ATP → Oxaloacetate + ADP + Pi. - Enzyme: Pyruvate carboxylase · 2. ** ...

Distinct metabolic flux modes through the tricarboxylic acid cycle in ...

The results of this study indicate that there is incorporation of one carbon from the anaplerotic CO2 assimilation catalysed by ...