Optimizing sgRNA position markedly improves the efficiency of ...

Increasing the targeting scope and efficiency of base editing with ...

... better editing microenvironment for BEs. Our results showed that editing efficiency could be markedly improved in this way. We also optimized ...

Comparison of the Feasibility, Efficiency, and Safety of Genome ...

Optimizing sgRNA position markedly improves the efficiency of CRISPR/DCas9-mediated transcriptional repression. Nucleic Acids Res. 2016, 44, e141. [Google ...

Optimization of Nuclear Localization Signal Composition Improves ...

Type V CRISPR–Cas12a systems are an attractive Cas9-alternative nuclease platform for specific genome-editing applications.

Fine-Tuning of Transcription in Pichia pastoris Using dCas9 and ...

Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression. Radzisheuskaya, Aliaksandra ...

Compact and highly active next-generation libraries for CRISPR ...

Optimizing sgRNA. 916 position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional. 917 repression. Nucleic Acids Research gkw583. 918.

CRISPR-Cas orthologues and variants: optimizing the repertoire ...

... markedly improved. With these principles in mind, SpCas9 has been adapted to a dimeric nuclease with a similar mode of binding, potentially ...

Optimizing sgRNA length to improve target specificity and efficiency ...

The CRISPR/Cas9 gene editing system has enhanced the development of genetically engineered animals for use in xenotransplantation.

CRISPR interference-based specific and efficient gene inactivation ...

Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression. Nucleic Acids Res. 44, e141 (2016). https ...

Complementary information derived from CRISPR Cas9 ... - Monash

... sgRNAs in total. To ... Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression.

A Comparative Study on the Efficiency of CRISPR/Cas9 Nuclease ...

Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression. Nucleic acids research. 2016;44(18):e141. 19 ...

S-EPMC3784321 < Europe PubMed Central < BioStudies < EMBL-EBI

Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression. S-EPMC5062975. ×. Doehn U. Doehn U.

A screening method to identify efficient sgRNAs in Arabidopsis, used ...

Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression. Radzisheuskaya, Aliaksandra ...

Targeted regulation of transcription in primary cells using CRISPRa ...

Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression. Nucleic Acids Res 44: e141 ...

CRISPR interference - Wikipedia

"Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression". Nucleic Acids Research. 44 (18): e141. doi ...

Dramatic Improvement of CRISPR/Cas9 Editing in Candida albicans ...

... optimize sgRNA expression and CRISPR ... Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional ...

Maximizing mutagenesis with solubilized CRISPR-Cas9 ...

Of note, appropriate adjustment of ionic strength with 300 mM KCl to solubilize and stabilize the reconstituted injection mixes markedly improves the ...

Method for CRISPR/Cas9 Mutagenesis in Candida albicans

requires 6 different gRNA oligonucleotides) because the efficiency of gRNA ... Optimizing sgRNA position · markedly improves the efficiency of ...

CRISPRmod CRISPRi all-in-one Lentiviral sgRNA - Horizon Discovery

Designs optimized from a published algorithm by Horlbeck, et. al. demonstrate strong levels of gene repression (see References tab); For genes with alternative ...

gRNA Design: How Its Evolution Impacted on CRISPR/Cas9 ... - MDPI

Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression. Nucleic Acids Res. 2016, 44 ...

Enhanced Efficiency of flySAM by Optimization of sgRNA ...

The location of GCs in sgRNAs did not affect activation efficiency. ... markedly improves the efficiency of CRISPR/dCas9-mediated ...