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Protein–DNA binding


Analysis of protein-DNA binding at equilibrium

Key words: protein-DNA interactions, binding curves, equilibrium binding, binding constant, dissociation constant, cooperativity, stoichiometry. Page 2 ...

DNA-Binding Proteins - Science

One protein (Cro) is a repressor of gene expression, the second (CAP) usually stimulates gene expression, and the third (lambda repressor) can act as either a ...

DNA Binding motifs overview - YouTube

This video describes several DNA binding motifs present in wide variety of Transcription factors and also describes how they interact with ...

Protein Dynamics and Contact Topology Reveal Protein–DNA ...

Although intrinsic protein dynamics do not seem to dictate the DNA-binding site, could they play a role in determining the docking orientation ...

Protein–DNA Interactions - Rooman - Major Reference Works

Further Reading. Banaszak LJ (2000) Foundation of Structural Biology. London, UK: Academic Press. Google Scholar. Branden C and Tooze J (1998) ...

Protein-dna binding - Vocab, Definition, and Must Know Facts

Protein-DNA binding refers to the interaction between proteins and DNA molecules, which is crucial for various biological processes such as gene regulation, ...

STAT protein, DNA binding domain (PF02864) - Pfam entry - InterPro

InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites.

EpiQuik General Protein-DNA Binding Assay Kit (Colorimetric)

The EpiQuik™ General Protein-DNA Binding Assay Kit (Colorimetric) is designed for measuring the transcription factor of DNA binding activity in nuclear extracts ...

DNA binding fluorescent proteins as single-molecule probes

DNA binding fluorescent proteins are useful probes for a broad range of biological applications. Fluorescent protein (FP)-tagging allows DNA binding ...

Purification of Sequence-Specific DNA-Binding Proteins by Affinity ...

complex migrates further in the gel. In addition, it is important to note that because the protein is radiolabeled, nonspecific DNA-binding complexes can be ...

USC researchers develop AI model that predicts the accuracy of ...

For understanding gene regulation, it is important to have access to the binding specificity of a protein to any DNA sequence or region of the ...

Predicting protein-DNA binding modes: Biophysical Journal

Transcriptor factor proteins bind DNA to regulate gene expression. Structural prediction of how they bind has been challenged by two ...

DNA-Protein Binding Assay Kit (Colorimetric) (ab117139) - Abcam

Assay DNA-Protein Binding in 3 hr with DNA-Protein Binding Assay Kit (ab117139). Cited in > 8 publications. Use with Microplate reader.

DNA-protein binding by fluorescence anisotropy | BMG LABTECH

Using fluorescence anisotropy titrations, we studied the effect of modified nucleotides on the recognition of DNA by the tumour supressor p53.

Computational design of sequence-specific DNA-binding proteins

The designed DBPs function in both Escherichia coli and mammalian cells to repress and activate transcription of neighboring genes. Our method ...

Balance between asymmetry and abundance in multi-domain DNA ...

These proteins are the most prevalent DNA-binding domain in eukaryotes and C2H2 type zinc-finger proteins (C2H2-ZFPs) constitute nearly one-half ...

Single-Stranded DNA Binding Protein - Promega Corporation

Single-Stranded DNA Binding Protein binds with high affinity to single-stranded DNA but does not bind well to double-stranded DNA.

Exploring Protein-DNA Binding Sites from Paired-End ChIP-Seq Data

Exploring Protein-DNA Binding Sites from Paired-End ChIP-Seq Data · Introduction · Data Set · Creating a MATLAB® Interface to a BAM Formatted File · Exploring ...

Modeling dependencies in protein-DNA binding sites

In this work, we explore Bayesian network representations of binding sites that provide different tradeoffs between complexity (number of parameters) and the ...

Investigation of various fluorescent protein–DNA binding peptides ...

Large DNA molecules were visualized with novel fluorescent protein–DNA binding peptides (FP–DBPs). We constructed FP–DBPs by linking fluorescent protein to ...