Quantification of hepatitis C virus RNA by competitive amplification ...

Stabilization of hepatitis C virus RNA by an Ago2–miR-122 complex

the 9.7-kb RNA genome detected in Northern blots) and the inability of the RT-PCR assay to discriminate between intact and partially degraded ...

Multiplex real-time quantitative RT-PCR assay for hepatitis B virus ...

Genomic amplification of one virus was unaffected by the simultaneous amplification of the other two. Competition between HCV and HIV-1 amplifications slightly ...

Technical Validation of a Hepatitis C Virus Whole Genome ...

DNA library preps made from RNA extracts of clinical samples from HCV-infected individuals with HCV Ct value <23 reproducibly resulted in more ...

HEPATITIS C DIAGNOSTICS TECHNOLOGY LANDSCAPE - Unitaid

The HCV amplification procedure begins with viral RNA ... blood specimens for evaluation of hepatitis C virus RNA and core antigen quantitative assays.

Evaluation of hepatitis C viral RNA persistence in HIV-infected ...

The aim of this study was to evaluate the persistence of HCV in HIV-coinfected patients with long-term SVR using ddPCR.

Hepatitis C Virus (HCV RNA Quantification Assay) - CDC

0 uses reverse transcription and PCR amplification primers that define a sequence within the highly conserved 5'-untranslated region of the. HCV genome. Sample ...

Spatiotemporal Analysis of Hepatitis C Virus Infection - PLOS

We have developed a single molecule RNA detection assay that facilitates the simultaneous visualization of HCV (+) and (−) RNA strands at the single cell level ...

Quantitative Branched DNA Assay and Genotyping for Hepatitis C ...

These findings suggest that the bDNA assay is a reliable test for HCV RNA. Detection of hepatitis C virus (HCV) RNA by reverse transcription?polymerase chain ...

Citations to Direct detection of circulating hepatitis C virus RNA ... - JCI

Quantitative detection of hepatitis C virus RNA with a solid-phase signal amplification method: Definition of optimal conditions for specimen collection and ...

Ultrastructural analysis of hepatitis C virus particles - PNAS

In this report, we generated an infectious HCV genome with an affinity tag fused to the E2 envelope glycoprotein. Using affinity grids, previously described to ...

Hepatitis C RNA, Quantitative - UW Laboratory Test Guide

The lower limit of quantitation is 10 IU/mL (1.00 log IU/mL). The limit of detection, defined as the minimum virus level that yields a positive result in 95% of ...

HCVQN - Overview: Hepatitis C Virus (HCV) RNA Detection and ...

Detection of acute hepatitis C virus (HCV) infection before the appearance of HCV antibodies in serum (ie, <2 months from exposure) Detection and ...

Laboratory Tests and Hepatitis C - Viral Hepatitis and Liver Disease

Most genotype assays rely on the amplification of short HCV RNA regions from clinical specimens, followed by a type-specific assay, such as restriction fragment ...

Modification of Glucose Metabolic Pathway to Enhance ... - MDPI

For mRNA analysis, the cell pellet was harvested at 8 h and 26 h from aliquots of 1 mL fermentation broth. The RNAprep Pure cultured cell/bacterial total RNA ...

Hepatitis C RNA Laboratory Procedure Manual - CDC

The quantitation of HCV viral RNA is performed using the HCV QS. The HCV QS compensates for effects of inhibition and controls the preparation and amplification.

Hep C Virus RNA Quantitative NAAT | Texas DSHS

Hep C Virus RNA Quantitative NAAT · Reliable results are dependent on adequate specimen collection, transport, storage, and processing. · Though rare, mutations ...